Agar plate

Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also done…

To begin, a streak was performed on two TSA plates and a blood agar plate using the three-sector streak technique from the unknown culture tube to isolate the two unknown bacterial colonies. The plates were then incubated for 12 hours at 37 ̊C. The blood agar plate was used to create a greater contrast in color to help distinguish between bacterial colonies by size and color. Two distinct colonies could be seen growing on the blood agar plate shown in figure 1. The smaller, opaque colored…

(GYP) agar medium (glucose- 1g, yeast extract- 0.1g, agar- 15 g, distilled water 1000mL and pH 6) containing 1 % soluble starch after 5 days incubation , the plates with fungal colony were flooded with 1% iodine and 2% potassium iodide. The appearance of clear zone surrounding the colony was considered positive for amylase enzyme. Protease activity Protease assay was performed by growing the endophytic fungi on GYP gar media amended with 1% skim milk and pH was adjusted to 6.5…

of the zones varied for each microorganism. Table 2 shows the results of the biochemical and morphological tests. The test that were performed were Sim, FTM, oxidase, catalase, MSA, blood agar, Acid fast stain and endospore stain. The sim and oxidase test were negative. The FTM, catalase, MSA and blood agar tests were positive. After the Acid Fast stain, the cells were blue and non-acid fast. After the Endospore stain, the cells were pink. This indicated that they were non-endospores.…

growth of Av. paragallinarum. Secondly, the media used in disc diffusion and broth dilution testing need to be validated. This validation process is completed by performing disc diffusion and broth dilution testing of quality control strain on candidate agar or broth. Then, the antimicrobial sensitivity of Av. paragallinarum can be tested on validated media, by both disc diffusion and dilution method. Any antibiotic resistance shown in the tests will be recorded and analysed. Finally, PCR will…

Wash hands and spray down or wipe your work are with disinfectant. Dry off with paper towels. Label the bottom of your agar plate (petri dish) as diagrammed in your Experimental Design (note: the bottom of the plate contains the nutrient agar, which looks like a clear/beige gelatinous material). Determine your two sources of collection for bacteria specimens. When you have decided, write them in tour data table on your lab report…

Oral Microbiome 10: Reflection 3 One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped…

Introduction Bacteriophages, informally known as phages, are a specific type of virus that only infect bacteria. Since their discovery in the early 1900s , researchers have began studying the various behaviors and interactions of phages within the environment (Poxleitner, Pope, Jacobs-Sera, Sivanathan, & Hatfull, 2016). Their name, bacteriophages, is derived from the idea that they are “bacteria eaters.” Due to their bacteria-destroying nature, they have been used in phage therapy in which they…

are not cross contaminated, label everything either Farm A, Farm B, or Farm C depending on what is being tested. There are six agar plates for each farm, three of the plates are tetracycline plates (which are labeled) and three are unlabeled. For the three labeled plates, mark each with their dilution which will be: 10-2 ,10-4, or 10-6; do the same for the unlabeled plates. Next, the dilutions were made using sterile technique so that unwanted bacteria is not introduced to the experiment.…

2.5. ANTIOXIDANT STATUS 2.5.1. TLC - Semi Quantitative DPPH Assay 0.2 % DPPH solution in methanol was prepared and kept in the fridge for further use. The grid space was marked with 1.0 cm2 space on an aluminum based TLC sheet (Merck silica gel 60F254) and a stock solution of all the extracts together with the standard were prepared in methanol. A series of dilutions of the stock together with the standard were prepared ranging from 400 µL to 0.01µL for the last dilution. The grid on the TLC…