Bacteriology

Now days, microorganisms are very important to identify to Genus and species because they can cause various diseases. We need to know that what helpful or harmful microbe in this environment. This project’s purpose is to identify an unknown bacterium by various methods. Most stains used in microbiology are differential stain, which use more than one dye so that different cells, chemicals, or structures such as Gram staining, TTC reduction, Oxygen requirement test, Catalase test, Nitrate test,…

Title The analysis of Mini unknown microbe# 1R Summary A series of tests was done with Microbe #1R for identification. A Gram stain was done it reveal a gram positive cocci result. A streak plate was completed revealing that the microbe was round/filamentous, convex, erose, granular, yellow, and opaque. The next test was the Tryptic Soy Agar plate for color. Followed by the oxidase test and last was the Mannitol Salt Agar test. Thus microbe #1R was Sporosarcina…

Biochemical Unknown I. Introduction Cultural Characteristics or morphology and biochemical tests can be used to identify and classify microorganism. By culturing microorganism in nutrient broth, slants, and on nutrient agar plates, the cultural characteristics or morphology can be determined. In this lab, the test tube 2 was incubated in a nutrient broth. The pigmentation of the tube was yellow. The media for test tube 2 was turbid. There were a few sediment on the bottom of the broth. In…

INTRODUCTION There have been multiple staining techniques before the Gram stain came about in 1883. These old staining techniques brought about issues because they stained both the tissue and the cells so it was hard to differentiate between the two. Christian Gram is the inventor of the gram stain and found this technique when he was looking at Streptococcus pneumoniae in the lung tissue of people and animals (1). When working with these tissues, he found that this bacterium would take up a…

After, then other biochemical tests were performed that were specific to the gram- negative bacteria to start the identification process of the unknown. The results of the gram negative tests lead to the identification of the bacteria Alcaligenes faecalis. The gram stain procedure was the first test performed by isolating a colony from the pure culture streak plate. The gram stain was followed orderly right from the referenced laboratory manual. The test resulted to be Gram- Negative bacteria…

The aim of this experiment is to identify and classify of an unknown bacterial isolate. Morphological and Biological test were preformed to identifiy the isolates characteristics. The following characteristics were tested: gram-stain, motility, morphology, hemolysis, lactose fermetation, mannitol fermentation, osmotolerance, aesculin hydrolysis, starch hydrolysis, gelatine hydrolysis, amino acid decarboxylation, lipid hydrolysis, lactose fermetation, casein digestion, catalase enzyme production,…

Gram positive: First, the bacteria sample is placed on a glass slide and heated only to the point of rendering it innocuous in terms of being infectious to the handler. Next, the bacteria sample is treated with a gentian violet-iodine solution for up to sixty seconds. The slide is then gently rinsed under clean water and the Gram solution is applied, which is a mixture of iodine and potassium iodide diluted in water. This step triggers a reaction to the gentian violet compound. Initially, the…

Sejaphala M.D. Student No. 62399543 Unique Number: 770819 Introductory Microbiology MIB2601 Assignment 02 Due Date: 13 April 2018 QUESTION 1 1. Bacteria is stained using a primary stain called crystal violet and then fixed with a mordant which in most cases is iodine. While some bacteria have the ability to retain the primary stain, others discolor when exposed to the acetone and alcohol (95%). Hence, the Gram stain divides bacteria into two groups- these bacteria are grouped…

When finding out unknown bacteria you have to go through several steps to figure out what it could be. I was given unknown bacteria number 28 and asked to run different testing to identify exactly what this bacterium is. I ran 6 test to determine my unknown. These test includes: Gram Stain, SIM, Sucrose, Citrate, MAC, and EMB. Each one of these test are different and they will give a positive or negative feedback which is how we will know which bacterium I have. A gram stain is one of the most…

Burkholderia pseudomallei also called as pseudomonas pseudomallei is an intracellular gram negative bipolar staining aerobic motile shaped rod with a safety pin appearance when seen microscopically measuring 25 micro metre in length and 0.4 -0.8 micro metre in diameter . It is capable of self population using flagella. In vitro , it requires a temperature of 40 degree and grows in an acidic environment fermenting glucose and galactose . It produces both exotoxin and endotoxins. It causes…