Bacteriology

INTRODUCTION This paper looks at the difference between gram negative and gram positive bacterium. This is important when doing gram staining experiments. It also looks at the importance of microbiology. DIFFERENCES OF GRAM NEGATIVE AND GRAM POSITIVE BACTERIUM There are notable differences between the gram negative and gram positive bacterium. Gram negative bacterium is surrounded by a thin peptidoglycan cell wall while Gram positive has a thick layer of peptidoglycan. The peptidoglycan around…

My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown, we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture, therefore we are very confident that it is correctly identified. We identified that K.…

A culture of different species was brought to the lab to be analyzed in order to identify the pathogen causing disease. The goal of this lab was to be able to identify the unknown pathogen, match the patient to the bacterium, and being able to identify the disease it causes. At the beginning of class, the TA handed each student an agar plate containing some unknown bacteria, each one was numbered. Mine was number 14. The teacher then asked the students to use the techniques used in class…

There were many tests performed on my unknown, which led me to conclude that the unknown microorganism is Pseudomonas fluorescens. When determining the gram reaction of the unknown, a Gram stain was done. This process differentiates between a Gram-positive and Gram-negative microorganism. This is done in four steps. The microorganism was stained with iodine, crystal violet, alcohol and acetone, and safranin. Iodine was used first as it acts as a mordant. It forms an insoluble complex by binding…

Investigating Enterococcus faecalis’s structures and resistances to antibiotics Introduction Bacteria’s phenotype is commonly described as gram-negative or gram-positive from conducting a gram stain. A bacterium that is gram-negative tends to have cells composed of a thin peptidoglycan layer, and a gram stain of gram-negative bacteria are seen as red or pink through a microscope (Smith and Hussey, 2005). On the other hand, a bacterium that is gram-positive will have cells made of a thick…

Week 3 of lab, we received our unknown microorganisms. That week we performed a differential staining to determine whether our unknown has a gram-negative envelope which stains red or gram positive envelope which stains blue. Gram-negative microorganisms stain red because of their inability to bind crystal violet and their ability to bind the safranin. Gram-positive microorganism, however, stains blue due to their ability to retain the primary stain, crystal violet. I determined my unknown…

the 20th century, society adapted it to pre-existing assumptions about ethnicity, race, and class. “The new discipline of bacteriology amassed persuasive evidence identifying particular microbes as responsible for such deadly diseases as cholera, tuberculosis, gonorrhea, typhoid fever, scarlet fever, and diphtheria (p. 234. Chapter introduction). This quote shows how bacteriology was giving a scientific reason for the worst diseases plaguing society. One would think that because “contagion could…

Marcus Ward Lyon Jr. studied mammalogy, bacteriology, and Pathology. Lyon was born on February 5, 1875 in Rock Island Arsenal, Illinois. For this time and being he had a fairly small family with only two siblings, Henry S. Lyon and James W. Lyon. His mother , Lydia Anna Lyon, and his father a military man named Captain Marcus Ward Lyon Jr. Because of his father’s military career, Lyon’s family moved across the United States throughout his childhood. When Lyon’s family was stationed at Watertown…

are four type of microorganisms that causes infectious disease are bacteria, parasites, fungi and viruses. Microbiology laboratory has many sections and each section do many types of tests. The microbiology laboratory has many sections such as bacteriology, parasitology, virology and mycology. The microbiology laboratory is study the microorganism that cannot be seen by the eye such as bacteria and fungi to medical diagnosis.…

Alexander Fleming was the genius of “technical ingenuity and original observation.” His efforts on bruise infection and lysozyme, a sterilizing enzyme found in tears and saliva, granted him a place in the history of bacteriology. Fleming work during WW 1 was against antiseptics on deep wounds, but most physicians did not trust his findings. Fleming’s work and diligence got him knighted in 1944. Sir Alexander Fleming was born August 6, 1881, in Ayrshire, Scotland. Sir Alexander Fleming lived at…