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  • T2 Bacteriophage

    of a bacteriophage is a vital component of microbiology. Bacteriophages are used for a number of scientific applications, including the decontamination of food products, human therapy, and much more, thus it is critical to determine the concentration of the bacteriophage in a solution. Doing so allows scientists to determine bacteriophage potency, side effects, and minimal effective doses for clinical applications (Andersen et al., 2011). This experiment allows scientists to estimate the number of bacteria present in a tube of T2 bacteriophage which will infect Escherichia coli strain B (E. coli). The results can be used to determine how effective the T2 bacteriophage is in halting the growth…

    Words: 768 - Pages: 4
  • Bacteriophage Experiment

    Introduction Bacteriophages, informally known as phages, are a specific type of virus that only infect bacteria. Since their discovery in the early 1900s , researchers have began studying the various behaviors and interactions of phages within the environment (Poxleitner, Pope, Jacobs-Sera, Sivanathan, & Hatfull, 2016). Their name, bacteriophages, is derived from the idea that they are “bacteria eaters.” Due to their bacteria-destroying nature, they have been used in phage therapy in which they…

    Words: 1340 - Pages: 6
  • Isolating Phage

    This study is about isolating phage. The first step was to do an enrichment isolation then do a plaque assay and spot test. After that the next protocol was do a plaque streak, which was conducted numerous of times. Then serial dilution was conducted and RE Digests of Phage DNA as well. Last step was protocol 10.3, gel electrophoresis. The relevance of this study is important to field of biology because bacterial infections could be treated with bacteriophages. Bacteriophages can be a solution…

    Words: 1761 - Pages: 8
  • Transformation Of Pglo Lab Report

    Transformation of pGlO plasmid to an E.coli bacteria cell We pipetted 250 ul of CaCl2 transformation solution to into two separate tubes. Next, we used a sterile loop to transfer two to four larger bacteria colonies from an E.coli plate into each of the two tubes. The bacteria was observed under UV light to make initial observations. We pipetted 10 ul of pGLO plasmid (0.08 ug/ul) into one of the tubes. Both tubes were incubated on ice for ten minutes. After ten minutes of incubation, the tubes…

    Words: 785 - Pages: 4
  • Bacteriophage Lab Report

    Introduction: Viral infections can affect many things, including bacteria. When bacteria undergo infections from a virus that virus is called a bacteriophage. After a bacteriophage infects a bacteria it can create two different phases, the lytic or lysogenic cycles. During a lytic stage the bacteriophage causes death to the bacteria. When this happens on an agar plate covered in bacteria it creates a clear spot known as a plaque. Each plaque is created by one virus so from the number of plaques…

    Words: 2224 - Pages: 9
  • Bacteriophage Phage Therapy (Disadvantages)

    Bacteriophages PROS (Advantages) Phage therapy has a lot of advantages more than antibiotics [39]. First of all, phages have a broad antibacterial activity even against antibiotic resistance bacteria [124, 125, 126] like MRSA as phage can be used as sanitizing agent against hospital acquired infections like MRSA [86]. Also a phage (pVp-1) was able to overcome the resistant strain of Vibrio parahaemolyticus in experimentally infected mice [127]. Phages can lyse bacteria in the supurrative…

    Words: 936 - Pages: 4
  • Similarities Between Alfred Hershey And Martha Chase

    Alfred Hershey & Martha Chase: DNA Discoveries Alfred Hershey was born in Owosso, Michigan. He graduated from Michigan State in 1934 with a Ph.D. After his Ph.D., Hershey accepted a position at the Washington University School of Medicine in the Department of Bacteriology, where he started working on bacteriophage. In 1946, working with Max Delbruck, a fellow scientist, Hershey discovered that phage can recombine when co-infected into a bacterial host, which lead to research a new area of phage…

    Words: 737 - Pages: 3
  • Biology Discourse Community

    Bacteriophage is a virus that attacks bacterial cells and “disrupts” its normal metabolism, causing “the bacterium to lyse” (Sulakvelidze et al., 2001). Its effect on bacteria make the bacteriophage a possible new treatment for antibiotic resistant superbugs. Golkar et al. (2013) discuss the various advantages bacteriophage therapy offers over antibiotics. They are “very specific to their hosts”, meaning they can be easily manipulated to detect a specific bacterial infection. Antibiotics, on the…

    Words: 1932 - Pages: 8
  • Gram Positive Cocci Case Study

    with an MIC equal to 0.25ug/mL, and imipenem with an MIC equal to 0.016ug/mL. Based on our collected data, we are able to conclude that either the patient in Case 3 has a Staphylococcus epidermidis infection or S. epidermidis is a contaminant in the blood bottles. Case 4 has several patients suspected of being involved in a hospital outbreak of S. aureus and we are interested in identifying the pattern of susceptibility to different bacteriophages. Knowing susceptibility patterns can elucidate…

    Words: 1409 - Pages: 6
  • Hershey-Chase Experiment

    She is also the first female scientist that I came across in a very male dominated field of genetics and biology. The Hershey-Chase experiment was one in 1952 (Dnaftb, n/a) where Hershey marked bacteriophage DNA and protein shells to prove that DNA was passed down and protein was not. Hershey knew that bacteriophages contained basically two parts, a protein shell, and DNA. He used a radioactive isotope of phosphorus (which phosphorous isn 't found in DNA) to "mark" the DNA of the…

    Words: 1577 - Pages: 7
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