Baglgc Bacteria

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The ΔglgC cyanobacteria will be transformed yet again to express an engineered new, small PSII antenna. In order to do this, plasmids pGDNG3/Cys(181/226)Ser and p613 will be co-transformed using the method described in the paper by Beckman et al. The method of using a vector containing an antibiotic (ampicillin) resistance gene will also be employed in this experiment. Ampicillin will then be added to the transformed bacteria in order to obtain the successfully transformed bacteria. By adding this engineered PSII antenna, the efficiency of light capture is increased. This experimental group will also be placed in nitrogen starved conditions and the photosynthetic (light) conditions will be identical to those in the control group. These mutant …show more content…
The elution that corresponds to the alpha-ketoglutarate will be separated from the rest of the biomass. Once the alpha-ketoglutarate is isolated, it can undergo an acid-base titration in order to determine its concentration. An auto-titration will be in order to get the most accurate midpoint. The necessary calculations and data analysis will be done to determine the final concentration present in the biomass. This can then be compared for all of the groups: the experimental ΔglgC cyanobacterial mutants and the control group that consists of just ΔglgC …show more content…
SYK-6. The muconic acid and adipic acid can be separated using using HPLC. Once both acids are separated, the muconic acid can undergo an acid-base titration in order to determine its concentration. Auto-titrations will be done on both acids and the data will then be analyzed so that the final concentration of each acid can be determined.
Since the cell density might vary among the different groups of bacteria, the amount of products produced should be reported as a percent mass. The concentration of each product can be converted to the total mass present. The mass of the total biomass from each bacterial group should be known in order to determine the percent mass. The results can then be compared and a conclusion can be

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