bilimbi fruits (ABE) was fractionated by 100% petroleum ether (F1), followed by ethyl acetate: petroleum ether (F2, 20 : 80), ethyl acetate: petroleum ether (F3, 40 : 60), ethyl acetate: petroleum ether (F4, 60 : 40), ethyl acetate: petroleum ether (F5, 80 : 20), 100% ethyl acetate (F6), methanol: ethyl acetate (F7, 20 : 80) and methanol: ethyl acetate (F8, 40 : 60).
Sl No Fractions Yield (g %)
F1 100% petroleum ether 1.2%
F2 ethyl acetate: petroleum ether (20 : 80) 4.9%
F3 ethyl acetate: petroleum ether (40 : 60) 19.4%
F4 ethyl acetate: petroleum ether (60 : 40) 24.3%
F5 ethyl acetate: petroleum ether (80 : 20) 17.8%
F6 100% ethyl acetate …show more content…
The percentage inhibitions of fractions were 2.36% (F1), 12.91% (F2), 21.58% (F3), 32.53% (F4), 87.50% (F5), 47.28% (F6), 40.22% (F7), 39.24% (F8) and standard gallic acid (GA) 74.60%. Ethyl acetate : petroleum ether (F5, 80 : 20) fraction exhibited superior inhibition of DPPH radical followed by F6, F7, F8, F4, F3, F2 and F1 fractions. F5 fraction shows superior DPPH radical scavenging activity than the standard gallic acid. Figure 2 DPPH radical scavenging assay
10.3.4 HPLC –DAD analysis
Polyphenolic compounds gallic acid, epicatechin, vanillic acid, chlorogenic acid, p-Coumaric acid, myricetin, quercetin, ferulic acid, cinnamic acid and kaempferol were used as standards. Potent F5 fraction of ABE is subjected to HPLC-DAD analysis showed the presence of myricetin, quercetin and p-coumaric acid. The yield of these phenolics from 100 g fresh Averrhoa bilimbi fruits are 151.60, 77.39, 246.21 mg/100g respectively. The results are presented in Figure 3 and 4 and the active principles with their retention time (RT), area, height, area% and height% are presented in Table 3 and