Factionation Of ABE Extract By Column Chromatography Case Study

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Natural products either as pure compounds or as standardized extracts provide unlimited chances for new drug discoveries because of the unmatched availability of chemical diversity (Cos et al., 2006). According to the World Health Organization (WHO), more than 80% of the world's population trusts on traditional medicine for their primary healthcare needs. Plants used for traditional medicine contain a varied range of compounds that can be used to treat chronic as well as infectious diseases (Duraipandiyan et al., 2006). Clinical trials are necessary to demonstrate the effectiveness of a bioactive compound to verify this traditional claim. Clinical trials are directed towards understanding the pharmacokinetics, bioavailability, efficacy, safety …show more content…
bilimbi fruits (ABE) was fractionated by 100% petroleum ether (F1), followed by ethyl acetate: petroleum ether (F2, 20 : 80), ethyl acetate: petroleum ether (F3, 40 : 60), ethyl acetate: petroleum ether (F4, 60 : 40), ethyl acetate: petroleum ether (F5, 80 : 20), 100% ethyl acetate (F6), methanol: ethyl acetate (F7, 20 : 80) and methanol: ethyl acetate (F8, 40 : 60).
Sl No Fractions Yield (g %)
F1 100% petroleum ether 1.2%
F2 ethyl acetate: petroleum ether (20 : 80) 4.9%
F3 ethyl acetate: petroleum ether (40 : 60) 19.4%
F4 ethyl acetate: petroleum ether (60 : 40) 24.3%
F5 ethyl acetate: petroleum ether (80 : 20) 17.8%
F6 100% ethyl acetate
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The percentage inhibitions of fractions were 2.36% (F1), 12.91% (F2), 21.58% (F3), 32.53% (F4), 87.50% (F5), 47.28% (F6), 40.22% (F7), 39.24% (F8) and standard gallic acid (GA) 74.60%. Ethyl acetate : petroleum ether (F5, 80 : 20) fraction exhibited superior inhibition of DPPH radical followed by F6, F7, F8, F4, F3, F2 and F1 fractions. F5 fraction shows superior DPPH radical scavenging activity than the standard gallic acid. Figure 2 DPPH radical scavenging assay
10.3.4 HPLC –DAD analysis
Polyphenolic compounds gallic acid, epicatechin, vanillic acid, chlorogenic acid, p-Coumaric acid, myricetin, quercetin, ferulic acid, cinnamic acid and kaempferol were used as standards. Potent F5 fraction of ABE is subjected to HPLC-DAD analysis showed the presence of myricetin, quercetin and p-coumaric acid. The yield of these phenolics from 100 g fresh Averrhoa bilimbi fruits are 151.60, 77.39, 246.21 mg/100g respectively. The results are presented in Figure 3 and 4 and the active principles with their retention time (RT), area, height, area% and height% are presented in Table 3 and

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