High performance liquid chromatography

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  • Analysis Of Acetol And Guaiacol

    by a high-performance liquid chromatography pump (Agilent) at rates between 0.06 to 0.17 ml/min. The evaporator set at a temperature of 180C allowed to ensure water was in vapor form before entering the reactor. Furthermore, the transport of the water vapor and liquid organic feed was assisted by the nitrogen gas that flowed through the same pipe. The syringe pump used for the organic feed injection was automatically controlled by a computer program according to set values. Because of the high…

    Words: 842 - Pages: 4
  • Lab Report Gas Chromatography

    Gas chromatography (GC) is a chromatography technique where the separation of individual components (called analytes) from a sample relies on their differing distribution between the mobile phase and a stationary phase. The mobile phase is what carries the analyte (components being analysed) through the stationary phase and in GC, it is an inert gas, (usually helium or nitrogen). The gas must be inert so it will not react with the samples to give a false reading. The stationary phase is the…

    Words: 1375 - Pages: 6
  • Mass Spectrometry

    interactions [X16]. The more recent inlet techniques (Lasterspray ionization inlet [LSII], matrix-assisted ionization inlet [MAII], and solvent-assisted ionization inlet [SAII]) are sensitive, useful at both high and low mass range and can be used at ambient pressure or with a vacuum (With liquid or tissue specimens too) [X16]. These inlet techniques are functionally similar to ESI in the spectra they produce, these techniques also inherit the delicate treatment of proteins from ESI too…

    Words: 1882 - Pages: 8
  • Liquid Chromatography Case Study

    Kate Byrne DT299/4 CHEM 4128 Applied Physical & Analytical Chemistry Assignment Liquid Chromatography High Resolution Mass Spectrometry Hyphenated Techniques The term “hyphenated technique” was introduced by Hirschfeld in 1980. It refers to the combination of a chromatographic separation technique and a spectroscopic detection technique to form a new, more efficent instrument. The technique which will be discussed in this case is LC-MS. The aim of combining both techniques is to purify and…

    Words: 1044 - Pages: 5
  • Analysis Of Losartan Potassium

    (II, 2-n-butyl-4-chloro-1-[2- (1H-tetrazol-5 yl) biphenyl- 4-yl) methyl] imidazole-5- carboxyl acid)[1]. The determination of Losartan has been carried out in tablets by HPLC, capillary electrophoresis and super-critical fluid chromatography[2,3], in urine by gas chromatography- mass spectrometry[4] and, simultaneously with its active metabolite in biological fluids, by HPLC[5- 10]. Amlodipine Besylate (ADB) )[FIG.1.B], chemically,…

    Words: 1564 - Pages: 7
  • Factionation Of ABE Extract By Column Chromatography Case Study

    Natural products either as pure compounds or as standardized extracts provide unlimited chances for new drug discoveries because of the unmatched availability of chemical diversity (Cos et al., 2006). According to the World Health Organization (WHO), more than 80% of the world's population trusts on traditional medicine for their primary healthcare needs. Plants used for traditional medicine contain a varied range of compounds that can be used to treat chronic as well as infectious diseases…

    Words: 845 - Pages: 4
  • Amoxicillin And Fluid Chromatography In Athlone Laboratories

    Chapter 1: Introduction 1.1 Nature of the Dissertation Identification, quantification and analysis Amoxicillin and Flucloxacillin Sugar free antibiotics which are manufactured at Athlone Laboratories by high liquid fluid chromatography (HPLC). The project also involves Method Transfer validation by analysis Amoxicillin Sugar Free for the new project of GSK Mayenne France, this involved Identification, quantification and analysis of carrying Amoxicillin Trihydrate API and Sodium Benzoate in…

    Words: 2533 - Pages: 11
  • Importance Of Protein Interaction

    Protein-protein interaction can be defined as the interactions between different protein molecules within the cellular organization leading to formation of large protein complex which is modulated by non-covalent interactions such as hydrophobic interaction, hydrogen bond, van der Waals interactions etc. Protein-protein interaction plays an important role in functioning of body’s significant mechanism such as replication, transcription, translation, signal transduction, cell cycle, etc. Thus,…

    Words: 1138 - Pages: 5
  • Coe Soft Case Study

    6. Casey DM. and Scheer EC. in 1993 reported the use of several surface conditioning agents on temporary soft lining material. The three different surface materials were treated with Poly ethyl methacrylate monomer, Minute-Stain glaze, or mono-poly glaze. These different samples were compared after 30 days. Samples were analyzed with the use of scanning electron microscope. Initially the surface of all samples was intact. Fewer irregularities were seen in monopoly and Minute-Stain glaze treated…

    Words: 738 - Pages: 3
  • Nitrite And Nitrate Contamination Of Surface And Ground Water Pollution

    death, because the nitrate is reduced to nitrite in the rumen by bacteria. Nitrite is highly toxic because it combines with hemoglobin and form methemoglobin which is enabling to carry oxygen. A death occurs within few hours after the ingestion of a high nitrate feed…

    Words: 5586 - Pages: 23
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