Aspergillus Case Study

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Aspergillus is a saprophytic ubiquitous fungi genus, which includes more than 200 species. This genus have positive and negative impact to human being. Some species of Aspergillus have pharmaceutical applications, but at same time, this genus could be pathogen of respiratory diseases, and also it could synthesys potent carcinogens. The most pathogenic specie of Aspergillus for human is A. fumigatus, which causes a wide range of sickness, depending to the immunological system of patient (Reference) .

On the other hand, there are several secundary metabolite synthesised by this fungi. For example, epoxysuccinic acid, difructosedianhydride, tryprostatins, cyclotrypostatins, spirotryprostatins, simple diketopioperazines, pyripyropenes,
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At the same time, its universality makes it unspecific and inconclusive regarding samples with great presence of the-same-type compounds. Then, in order to designate a structure to the analyte, another technique is required. In this case, that is achieved with MS coupling to the system.

Advantages on the coupling of DAD-MS

MS characteristics

Mass spectrometry
This technique is useful, because it help us to study masses of molecules or fragments, helping us to measure the mass to charge. In general, the mass spectrometer have a ionizer, vacuum pump, ion accelerator, could have magnets or/and electric field for separate ions , an analyzer tube, and an electron multiplier detector (see figure #).

Figure #.- Mass spectrometer.

There are different mass spectrometers, for example, transmission quadrupole mass spectrometer, time-of-flight mass spectrometer, quadrupole ion-trap mass spectrometer and ion mobility spectrometer. In this project, we are focussed in the time-of-time mass spectrometer, because our sample has been analyzed by it. This kind of spectrometry is a high-resolution spectrometry, it means that it could sense deltas of m/z of 0.0005. So, it is used to accurate mass measurement.

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