Risperidone (RIS), an atypical neuroleptic drug, reported to have fewer adverse effects than traditional agents, is effective in psychoses, such as schizophrenia, and other psychiatric illnesses in adults and children, including pervasive developmental disorders (PDD), autism, and attention-deficit disorder (ADD) [1–3]. It acts primarily by selective antagonism of dopamine Type 2 (D2) and serotonin Type 2 (5HT2) receptors in the brain [4–6]. Apart from minor N-dealkylation, the major pathway of biotransformation of RIS is hydroxylation at the 9_ position on the pyrido-pyrimidone ring to 9-hyroxyrisperidone (9-OH-RIS), mediated by the liver enzyme, CYP2D6 [7].
Risperidone is a second-generation antipsychotic agent widely used in the treatment of schizophrenia. It is converted to the active metabolite, 9-hyroxyrisperidone, in vivo. In fact, the “active …show more content…
However, lack of sensitivity hindered the application of LC-UV on the pharmacokinetic study of risperidone. Furthermore, requirement of large amounts of samples and long analysis time also restricted its application. LC-ECD was another choice for the quantification of risperidone [20-21]. But there were many practical issues, such as long time for stabilizing the detector, susceptible to be interfered by endogenous compounds, and restriction of mobile phases, and so on. LC–MS and LC–MS-MS methods have also been developed. Most of these methods need more than 0.5 ml of plasma or are less sensitive. Moreover, all these reports did not evaluate the measurement uncertainties of their method, which is an important parameter in
Risperidone is a second-generation antipsychotic agent widely used in the treatment of schizophrenia. It is converted to the active metabolite, 9-hyroxyrisperidone, in vivo. In fact, the “active …show more content…
However, lack of sensitivity hindered the application of LC-UV on the pharmacokinetic study of risperidone. Furthermore, requirement of large amounts of samples and long analysis time also restricted its application. LC-ECD was another choice for the quantification of risperidone [20-21]. But there were many practical issues, such as long time for stabilizing the detector, susceptible to be interfered by endogenous compounds, and restriction of mobile phases, and so on. LC–MS and LC–MS-MS methods have also been developed. Most of these methods need more than 0.5 ml of plasma or are less sensitive. Moreover, all these reports did not evaluate the measurement uncertainties of their method, which is an important parameter in