TLC and HPTLC
Introductions
Thin layer chromatography (TLC) is derived from liquid chromatography where liquid serves as mobile phase and thin layer of substance on a flat late surface serves as stationary phase. The thin layer of materials act as sorbent while the mobile phase known as solvent. Solvent will send the solutes through the sorbent depends on the strength of the mobile phase as it dissolved the solute and send them up the plate against gravity ,and resistance of sorbent when it attracts the solute out from the solvent[4]. The molecule in the solute moves in a stop-and-go motion as they are repeatedly absorbed and desorbed along the plate. Therefore, only small amount of solute are moving at a time and have traveled …show more content…
TLC techniques mostly involves adsorption and partition due to hydrogen bonding and dipole-dipole interaction. Adsorption TLC is the most sensitive techniques to separate compound that differences in polarity. However, partition based techniques will separate compound based on …show more content…
HPTLC contains all chromatographic techniques that use planar open stationary phase. Basic steps of HPTLC are fully automated and controlled by computer now. Different instrument have been developed for each steps such as sample applicators, developing chambers, scanning densitometers, and electronic documentation systems software. Advantages of HPTLC include all components of sample can be identified on a single plate and additional information of the sample can be obtained through further actions such as redeveloping, derivation and redetection. HPTLC can evaluate only the targeted zones without performing a complete experiment. Therefore, sample evaluations of partial zones can be fast and cost
Introductions
Thin layer chromatography (TLC) is derived from liquid chromatography where liquid serves as mobile phase and thin layer of substance on a flat late surface serves as stationary phase. The thin layer of materials act as sorbent while the mobile phase known as solvent. Solvent will send the solutes through the sorbent depends on the strength of the mobile phase as it dissolved the solute and send them up the plate against gravity ,and resistance of sorbent when it attracts the solute out from the solvent[4]. The molecule in the solute moves in a stop-and-go motion as they are repeatedly absorbed and desorbed along the plate. Therefore, only small amount of solute are moving at a time and have traveled …show more content…
TLC techniques mostly involves adsorption and partition due to hydrogen bonding and dipole-dipole interaction. Adsorption TLC is the most sensitive techniques to separate compound that differences in polarity. However, partition based techniques will separate compound based on …show more content…
HPTLC contains all chromatographic techniques that use planar open stationary phase. Basic steps of HPTLC are fully automated and controlled by computer now. Different instrument have been developed for each steps such as sample applicators, developing chambers, scanning densitometers, and electronic documentation systems software. Advantages of HPTLC include all components of sample can be identified on a single plate and additional information of the sample can be obtained through further actions such as redeveloping, derivation and redetection. HPTLC can evaluate only the targeted zones without performing a complete experiment. Therefore, sample evaluations of partial zones can be fast and cost