High performance liquid chromatography

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  • Cftriaxone And Tazobactam Lab Report

    “DEVELOPMENT AND VALIDATION OF STABILITY INDICATING METHOD FOR SIMULTANEOUS ESTIMATION OF CEFTRIAXONE AND TAZOBACTAM INJECTION USING RP-UPLC METHOD” 2.1 OBJECTIVE To develop and Validate Stability Indicating Method for simultaneous estimation of Ceftriaxone and Tazobactam in their combined dosage form by RP-UPLC method in various stress condition like, alkaline, acidic, oxidative, thermal and photo degradation. 2.4 METHODOLOGY 2.4.1 Chromatographic Condition Column Separation was achieved by…

    Words: 739 - Pages: 3
  • Gel Filtration Chromatography Lab Report

    Observe Ligand-Protein Interaction using Gel Filtration Chromatography (Phenol Red Binding to Bovine Serum Albumin) Introduction: Serum albumin is often known as blood albumin, and is the most abundant protein in humans and other animal plasma. Serum albumin is also known as a carrier protein by non-specifically binding several biomolecules. It is essential for maintaining the osmotic pressure that is needed for body fluids distribution between body tissues and intravascular compartments.…

    Words: 910 - Pages: 4
  • Difference Between Absorption And Desorption

    Adsorption is the bond of particles, particles, or atoms from a gas, fluid, broken down and attached strongly to a surface. This procedure makes a film of the adsorbate on the surface of the adsorbent. This methodology varies from absorbtion, in which a liquid (the absorbate) pervades or is absorbed by a porous solid. Adsorption is a surface-based procedure while absorption includes the entire volume of the material. The term sorption envelops both methodologies, while desorption is the converse…

    Words: 1425 - Pages: 6
  • Analysis Of Araliae Continentalis Radix

    acid; Phase I; Pharmacokinetics; Ultra-performance liquid chromatography-tandem mass spectrometry Abbreviations: AIC, akaike information criteria; AUC0-∞, the area under the concentration–time curve from zero to infinity; CL/F, the total body clearance for oral administration; Cmax, the maximum plasma concentration; CV, coefficient of variation; HPLC, high-performance liquid chromatography; ka, the absorption rate constant; IS, internal standard; LLE, liquid–liquid extraction; LLOQ, lower limit…

    Words: 972 - Pages: 4
  • Structural Biology Reflection

    purification of the protein under the guidance of Dr. Raquel Ruiz and involved expression tests at different concentrations of the inducing agent IPTG, large-scale growth of BL21-DE3 E. coli cells that over-express hS100A7, utilizing fast protein liquid chromatography (FPLC) with immobilized metal ion affinity and size exclusion columns, and ultimately using the purified protein product in setting up crystallization screens. I was unable to obtain any crystals of hS100A7 due to the usage of the…

    Words: 1169 - Pages: 5
  • Gas Chromatography Research Paper

    Part A Gas Chromatography What is it? Gas chromatography is a variation of chromatography used in analytical chemistry for separating and analysing components that can be vaporised without decomposition ("Gas Chromatography", 2016). This technique involves using a gas as the mobile phase. There are two types of gas chromatography: • Gas-solid chromatography – separates substances on the basis of their different strengths of adsorption on the solid • Gas-liquid chromatography – separates…

    Words: 2119 - Pages: 9
  • Diclofenac Synthesis Lab Report

    The researchers compared both biologically produced manganese oxides and chemically produced manganese oxides for the oxidation of diclofenac. Because biologically produced manganese oxides are known to have high specific surface areas and high catalytic reactivity, this substance offers higher rates of oxidation for diclofenac when compared to chemically produced manganese oxides.² If biologically produced manganese oxides prove to completely eliminate diclofenac from the water supply, the…

    Words: 1672 - Pages: 7
  • Fluid Chromatography Essay

    IV: Analytical applications I: What can be analyzed? At present, Supercritical Fluid Chromatography is very useful in this industries. And it has been used in the many fields. Pharmaceutical: It’s used in the drug industries to process the compound relate to the health and life as a vitamins, steroids, antibiotics, barbiturates, taxol, prostaglandins, non-steroidal anti-inflammatory agents, or etc. Moreover, it has been used for the achiral separations and purifications of substance in…

    Words: 853 - Pages: 4
  • Aspergillus Case Study

    time-of-flight mass spectrometer, quadrupole ion-trap mass spectrometer and ion mobility spectrometer. In this project, we are focussed in the time-of-time mass spectrometer, because our sample has been analyzed by it. This kind of spectrometry is a high-resolution spectrometry, it means that it could sense deltas of m/z of 0.0005. So, it is used to accurate mass measurement.…

    Words: 998 - Pages: 4
  • Tlc Lab Report

    TLC and HPTLC Introductions Thin layer chromatography (TLC) is derived from liquid chromatography where liquid serves as mobile phase and thin layer of substance on a flat late surface serves as stationary phase. The thin layer of materials act as sorbent while the mobile phase known as solvent. Solvent will send the solutes through the sorbent depends on the strength of the mobile phase as it dissolved the solute and send them up the plate against gravity ,and resistance of sorbent…

    Words: 728 - Pages: 3
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