Separation process

Title: Separations of a Mixture Notes: - H¬2O, Copper Sulfate, Starch - H2O (water)- liquid, solvent, boiling point: 100 C, freezing point: 0 C. - Copper Sulfate- solid, soluble, forms an aqueous solution, is a salt. - Starch- solid, macromolecule (long chain sugar), not very soluble in water at low temperatures. - When H2O, Copper Sulfate, and starch are mixed together, it forms a heterogeneous mixture. - Aqueous solution of copper sulfate with a suspension of starch. Research Question: What effect does the use of the separation techniques of filtration for the separating of the starch and evaporation for separating the water, have on the mass of the remaining substance, copper sulfate? Hypothesis: If a mixture of water, copper sulfate,…

Chromatography is a method of separation in which the components of a substance are separated and distributed between two phases, which are the mobile phases and stationary phase (1). There are multiple types of chromatograph, which includes paper chromatography, column chromatograph, retention chromatography, gas chromatography, liquid chromatography etc. Each technique has its own strengths and weaknesses, but all are useful in getting separations of different components (8). Since many…

The human body produces various essential proteins that provide functionality in the biochemistry of our system. How individual molecules are separated or a mixture of molecules are separated can be dependant of their size. Size exclusion chromatography (SEC), is the controlled separation technique that is based on the size of the particles in relation to the pore size (Barth and Saunders 2012). The separation process veers to be quick for large molecules and slower for smaller molecules. The…

Chromatography is a process used to separate molecules on the basis of chemical or physical properties, such as molecular mass, charge, or hydrophobicity. (Ninfa 121) There are many types of chromatography processes that can be used in different experiments and scenarios. These chromatography processes on include Gel filtration, ion exchange, and high pressure liquid chromatography. However, in this laboratory session the process of affinity chromatography is utilized to purify a His-tagged…

High Performance Liquid Chromatography Lab Abstract High performance liquid chromatography or HPLC is the process in which mixtures are physically separated according to their polarity. The lab conducted, the separation of dyes in grape Kool-Aid, demonstrated the concept. This was done to display how different alcohols and their concentrations have different polarities, in which ties into HPLC. The lab consists of testing Kool-Aid, water, and different concentrations of methanol and…

High performance liquid chromatography (HPLC) is a separation technique utilizing differences in distribution of compounds in two phases; called the stationary phase and the mobile phase. The stationary phase designates a thin layer created on the surface of fine particles and the mobile phase designates the liquid flowing over the particles. Under a certain dynamic condition, each component in a sample has a different distribution equilibrium depending upon the solubility in the phases and or…

Objective The objective of the experiment was to determine the purity and components of a mixture of fluorescein and bromonitroindoline, as well as separate them, through the process of thin layer chromatography. Procedure Part A- Separation of Compounds by Thin Layer Chromatography • Three 100 mL beakers and watch glasses were cleaned in preparation for this experiment. Three silica gel plates were acquired from the instructor, and to each plate a line was drawn 5 mm from the edge of the…

Introduction Extraction involves the separation of compounds in a mixture. This separation in based on the differences in each compound’s solubility. For the purpose of this lab, the liquid-liquid extraction technique was used. This method involves using two immiscible solvents, and because of each compounds solubility in each of those solvents, the mixed compounds can separate. Generally, this process includes water as one of the immiscible solvents, but for this lab, t-butyl methyl ether was…

Purpose: We already knew that the chemical properties of each pure substance are not are not changed after separation in a mixture. The key to find a physical property that one part of the mixture does have but the parts of the mixture doesn’t. In magnetism a magnetically susceptible material is extracted from a mixture depending on a magnetic force. Filtration is separation of an insoluble solid and a liquid. Filtration depends on solubility, the filtrate is the one want to keep (liquid) and…

Thin Layer chromatography was also use to separate the mixture of fluorene and fluorenone. Thin layer chromatography is a useful separation method when the components of a mixture are in question. Analyzing what the mixture consist of can be efficiently determine by TLC by calculating Rf and comparing these values. For the separation and isolation of a mixtures components, this method is extensive and not as efficient at column chromatography. This conclusion is represented in the data gathered.…