High performance liquid chromatography

1. Abstract In this study, reverse micellar extraction of asparaginase model system was carried out using CTAB/Iso-octane/hexanol/butanol system to optimize the forward and back extraction efficiency. By using Taguchi’s orthogonal array (OA), maximum forward extraction efficiency of 55.0%, 61.0% and 54% was achieved with an aqueous phase pH of 11.0, 150mM CTAB/Iso-octane and 0.1 M NaCl, respectively. Similarly, Taguchi’s orthogonal array (OA) was applied to optimize the pH of stripping phase,…

This experiment monitored the synthesis of uridine from the deamination of cytidine through separating the contents of the reaction mixture using HPLC (High-performance liquid chromatography) at various time intervals since the start of the reaction. Cytidine and uridine standards are ran and the output of the HPLC will show two peaks based on the retention times of each compound. Identified the peak with the retention time of 2.422 minutes is cytidine and uridine has a retention time of 2.716…

the peptide will be submitted to the URMC Proteomics Resource and Laboratory for analysis using LC-MS/MS tandem mass spectrometry. In this approach, the peptides will be separated and introduced into a mass spectrometer using reversed phase liquid chromatography (RC-LC). This spectrometer will measure the mass and charge of each peptide. Then, the peptide will be isolated and fragmented along the backbone to generate tandem of mass spectrum. The peptide MS/MS spectra will then be searched…

Protein Purification Practical Report Student Name : Noel Murphy Date : 28 February 2015 UF/DF Practical Question Report Ultrafiltration (UF) and Diafiltration (DF) steps are carried out prior to every chromatography step and post the final polishing chromatography step. These UF and DF steps are vitally important, the UF step concentrates the protein product into a smaller volume of buffer, while the DF step alters the conditions the protein product is sitting in i.e. Buffer exchange.…

My proclivity towards Pharmaceutical Sciences, especially for Drug interaction with the human body, became intense when I met my uncle who is a pharmacist and was visiting to advise my father for his medications. At that point, I was in 9th grade. I was curious how these chemicals can heal human body and help people to get healthy. I realized that I wanted to delve deeper into the subject that helped my father to recover from his illness. I made up my mind to pursue a career in the…

and inhibit South Africa, Asia, and Australia as an ornamental, for camel feed (Brutsch and Zimmermann, 1993) and for its delicious fruits as food for humans, Vervet monkeys and baboons. O. ficus is succulent shrub or tree reaches to 1.5- 3 meters high which may be reached to 5 meters, propagated by seeds or seedlings. Woody trunks become stiff with age, have flat branches called Cladodes afford spines or may…

the pomace is mixed with hot water and liquefaction enzymes (pectinases and cellulases). The pomace is then pressed once more for extraction juice41-43. This process results in the release of oligo- and polysaccharides from the apple pomace, causing high colloid concentration in the extraction juice43. Some of oligosaccharides mentioned above were studied for their beneficial physiologic effects, such as potential prebiotic activities38, as potential chemoprevention agent or anti-tumor agent by…

dry leaf, fresh barks of plant, induced callus, somatic embryo and suspension culture (In exogenous medium and in pallet) in 100 mg/ml were analyzed for CPT content by TLC (Thin Layer Chromatography) and HPLC (High Performance Liquid Chromatography). TLC and HPLC analysis confirm the presence of CPT in dry leaf, fresh barks of the plant, induced callus and somatic embryo. Out of which bark extracts possessed a maximum amount of…

lack of structural details limits the study of their potential applications. A rapid and effective method for identifying oligosaccharides is desirable yet the complete structural characterization of novel oligosaccharides remains challenging. The high polarity nature and highly branching, isomeric structures makes chromatographic separation with typical reversed-phase HPLC columns generally difficult. Moreover, oligosaccharide structures are usually in great diversity and the comprehensive…

fraction, 4-5 drops of Hager’s reagent (saturated solution of picric acid) was added and observed for the presence of yellow precipitate formation. Vinblastine and methanol was used as positive and negative control respectively. 4.5 Thin Layer chromatography…