Chromatographic Separation Of Oligosaccharide Movements

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Oligosaccharide structures are strongly associated with their potential biological activities. Despite the biological activities of oligosaccharides have known for years, the lack of structural details limits the study of their potential applications. A rapid and effective method for identifying oligosaccharides is desirable yet the complete structural characterization of novel oligosaccharides remains challenging. The high polarity nature and highly branching, isomeric structures makes chromatographic separation with typical reversed-phase HPLC columns generally difficult. Moreover, oligosaccharide structures are usually in great diversity and the comprehensive structural characterization requires the determination of the degree of polymerization, the sequence of the constituent monosaccharide units, the numerous linkages, the position of branching and possible presence of isomers51-52. Additionally, oligosaccharides lack specific chromophores in the ultraviolet-visible (UV) region suitable for the UV detection. Therefore, the exploration of oligosaccharide structures requires a combination of several analytical methods.
Chromatographic separation techniques
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Separation of oligosaccharides appears essential to investigating complex oligosaccharide structures from plant origin. The chromatographic separation has been carried out in GC and HPLC using many types of stationary phase. The most commonly used technologies include hydrophilic interaction chromatography (HILIC), high performance anion exchange chromatography (HPAEC), and HPLC with porous graphitized carbon (PGC) column. Table 1.3 compares the advantages and disadvantages of some chromatographic separation techniques on oligosaccharides

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