Chromatographic separation techniques …show more content…
Separation of oligosaccharides appears essential to investigating complex oligosaccharide structures from plant origin. The chromatographic separation has been carried out in GC and HPLC using many types of stationary phase. The most commonly used technologies include hydrophilic interaction chromatography (HILIC), high performance anion exchange chromatography (HPAEC), and HPLC with porous graphitized carbon (PGC) column. Table 1.3 compares the advantages and disadvantages of some chromatographic separation techniques on oligosaccharides