Opuntia Ficus Case Study

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Introduction
Opuntia ficus-indica (L.) Mill belongs to the family Cactaceae. Its globally common name is the sweet prickly pear locally is called Albarshom. It has widely international spread. Firstly, Opuntia ficus-indica has founded Central America (Mexico). And later the species have been introduced to many areas of savanna regions and inhibit South Africa, Asia, and Australia as an ornamental, for camel feed (Brutsch and Zimmermann, 1993) and for its delicious fruits as food for humans, Vervet monkeys and baboons. O. ficus is succulent shrub or tree reaches to 1.5- 3 meters high which may be reached to 5 meters, propagated by seeds or seedlings. Woody trunks become stiff with age, have flat branches called Cladodes afford spines or may
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This may include some mandatory control measures. The study also aims at the effect of different heights on the genetic diversity of plants O. ficus and the study of biodiversity within this species. Where it is important in the ecosystem of the reserve. Using a technique inter-simple sequence repeat (ISSR) (Spooner,2005). This technique applied genetic identity study of O. ficus and useful for taxonomic studies of closely related O. ficus species. In addition, ISSRs involving gene mapping studied of the O. ficus. ISSRs are DNA fragments located inversely oriented. DNA segments of about 100-3000 bp amplified by PCR and flanked by closely spaced microsatellite sequences (DNA repeated sequence are short in specific locus) by a single primer or a pair of primers. ISSR is fast, effective and highly informational technique. They are considered reliable for DNA fingerprinting and by using Random Amplified Polymorphic DNA (RAPD) technique can be recognized to detect any genetic changes at the individual level as well as to clarify any close relationship between O. ficus species. To consider also their importance to fill gaps of gene …show more content…
A., and Kraaij, T. (2014). Alien flora of the Garden Route National Park, South Africa. South African Journal of Botany,94, 51-63. Brutsch, M. O., and Zimmermann, H. G. (1993). The prickly pear (Opuntia ficus-indica [Cactaceae]) in South Africa: Utilization of the naturalized weed, and of the cultivated plants. Economic Botany, 47(2), 154-162.
Cowling, R. M., Richardson, D. M., and Pierce, S. M. (Eds.). (2004). Vegetation of southern Africa. Cambridge University Press.
Ennouri, M., Ammar, I., Khemakhem, B., and Attia, H. (2014). Chemical composition and antibacterial activity of Opuntia ficus-indica F. Inermis (Cactus Pear) Flowers. Journal of medicinal food, 17(8), 908-914. Heuzé, V., Tran, G. (2015). Prickly pear (Opuntia ficus-indica). Feedipedia, a programme by INRA, CIRAD, AFZ and FAO. https://www.feedipedia.org/node/120 Last updated on October 2, 2015, 15:14
Lee, E. H., Kim, H. J., Song, Y. S., Jin, C., Lee, K. T., Cho, J., and Lee, Y. S. (2003). Constituents of the stems and fruits of Opuntia ficus-indica var. saboten. Archives of pharmacal research, 26(12), 1018-1023.
Spooner, D. (2005). Molecular markers for genebank management (No. 10). Bioversity

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