Reaction scheme of the deamination of cytidine to form uridine
Scheme 2. Mechanism of the deamination of cytidine using NaNO2 in the presence of acetic acid to form uridine.
The lab showed the successful deamination of cytidine to uridine as monitored by HPLC. The reaction progressed slower at room temperature relative to the same reaction carried out at 30oC. This is supported by the estimated t1/2 at room temperature = 74.5071 minutes and k= 0.0093 compared to at 30oC t1/2=74.5071 minutes and k=0.0287. At physiological temperatures, the reaction rate is estimated to be 0.1316, thus the same reaction would proceed even faster at more elevated