High performance liquid chromatography

then be analysed by HPLC (high-performance liquid chromatography) to determine quantitatively the concentration of the drug. The intrinsic dissolution rate will be determined using a stationary disk apparatus. The dissolution test will be performed using a USP apparatus 2 (paddle).The temperature will be set at 37◦C and rotation speed at 100rpm will be applied (United States Pharmacopeia 2015). The drug will then be extracted from the mixed media using the liquid-liquid extraction (Ghazal et al.…

the central nervous system.4 However, due to the relatively ubiquitous use of Papaveris Pericarpium in traditional Chinese medicines, its clinic safety has attractive significant attention for MOR and COD related toxicities. For example, MOR has a high potential for addiction, and its tolerability develops rapidly by frequent administration. In addition, MOR and COD have several severe side effects, such as nausea and vomiting, pruritus, constipation, urinary retention, respiratory depression,…

death, because the nitrate is reduced to nitrite in the rumen by bacteria. Nitrite is highly toxic because it combines with hemoglobin and form methemoglobin which is enabling to carry oxygen. A death occurs within few hours after the ingestion of a high nitrate feed…

in food quality control. Widely employed assays for xanthine oxido reductase activity are spectrophotometric quantitation of uric acid, the final product of the reaction [20] quantitation of hypoxanthine, xanthine or uric acid by high performance liquid chromatography (HPLC)…

Several methods have been described in the literature for the determination of MNT in its pharmaceutical dosage forms or in biological samples. Examples of these methods in biological samples are gas chromatography – mass spectrometry (GC-MS) [18] in plasma and urine of rats and human while GC-FID [19] was used for MNT analysis in human urine. Also, MNT in mixture with other drugs have been assayed in human plasma using GC-FID [20]. In pharmaceutical preparations…

emulsification activity of biosurfactants. Pseudomonas aeruginosa had higher emulsification activity than Saccharomyces cerevisiae. While checking the sugar and lipid content in biosurfactants by phenol sulphuric acid method and vanillin reagent method, found high in Pseudomonas aeruginosa than Saccharomyces cerevisiae. Under this study, after centrigugation of crude extract,the pellets were suspended in a 40 mL of dichloromethane in a separating funnel and shaken vigorously and the allowed…

women and children, acetaminophen is available over the counter in many forms. When consumed over long periods of time or in high dosages, acetaminophen has serious side effects including liver damage and other organ damages, therefore an effective quality control method of analysis. Here a method using cyclic voltammetry to analyze the concentration of acetaminophen in a liquid suspension Publix brand children’s pain reliever was developed and validated. A linear relationship from 0.79 ppt to…

drug and according to health 24 has the highest per capita Mandrax abuse in the world. When dugs are manufactured illegally the main aim, is produce a substance that is highly addictive, this creates dependence which at the end keeps the drug at a high consumer demand. This means that they are lots of impurities that are added to the drug to increase an individual’s dependency to the drug. In South Africa , and other countries in Asia that are still manufacturing Mandrax, the main impurity that…

to test manufacturing efficiency of two different types of polycaprolactone (PCL) with different average molecular weight. I concluded that the low molecular weight PCL is more convenient for mold-casting fabrication, due to low viscosity, while the high molecular weight PCL has an advantage of being mechanically stronger than the brittle…

part of an active collaboration with Dr. Tilla S. Worgall and/or in the Laboratory of Toxicology and HPLC of the Department of Clinical Laboratories, Pontificia Universidad Católica de Chile. Sphingolipid levels will be quantified by high performance liquid chromatography-triple quadrupole-tandem mass spectrometry (HPLC-MS/MS) on an Agilent 1200 HPLC system, equipped with an Agilent C18 column as described in protocol implemented and validated by our group2. Sphingolipid profiles will include…