High performance liquid chromatography

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  • Mass Spectrometry Lab Report

    the peptide will be submitted to the URMC Proteomics Resource and Laboratory for analysis using LC-MS/MS tandem mass spectrometry. In this approach, the peptides will be separated and introduced into a mass spectrometer using reversed phase liquid chromatography (RC-LC). This spectrometer will measure the mass and charge of each peptide. Then, the peptide will be isolated and fragmented along the backbone to generate tandem of mass spectrum. The peptide MS/MS spectra will then be searched…

    Words: 824 - Pages: 4
  • Pharmd Program Personal Statement

    My proclivity towards Pharmaceutical Sciences, especially for Drug interaction with the human body, became intense when I met my uncle who is a pharmacist and was visiting to advise my father for his medications. At that point, I was in 9th grade. I was curious how these chemicals can heal human body and help people to get healthy. I realized that I wanted to delve deeper into the subject that helped my father to recover from his illness. I made up my mind to pursue a career in the…

    Words: 772 - Pages: 4
  • Opuntia Ficus Case Study

    and inhibit South Africa, Asia, and Australia as an ornamental, for camel feed (Brutsch and Zimmermann, 1993) and for its delicious fruits as food for humans, Vervet monkeys and baboons. O. ficus is succulent shrub or tree reaches to 1.5- 3 meters high which may be reached to 5 meters, propagated by seeds or seedlings. Woody trunks become stiff with age, have flat branches called Cladodes afford spines or may…

    Words: 1096 - Pages: 5
  • Importance Of Polysaccharides In Apple Pomace

    the pomace is mixed with hot water and liquefaction enzymes (pectinases and cellulases). The pomace is then pressed once more for extraction juice41-43. This process results in the release of oligo- and polysaccharides from the apple pomace, causing high colloid concentration in the extraction juice43. Some of oligosaccharides mentioned above were studied for their beneficial physiologic effects, such as potential prebiotic activities38, as potential chemoprevention agent or anti-tumor agent by…

    Words: 930 - Pages: 4
  • Chromatographic Separation Of Oligosaccharide Movements

    lack of structural details limits the study of their potential applications. A rapid and effective method for identifying oligosaccharides is desirable yet the complete structural characterization of novel oligosaccharides remains challenging. The high polarity nature and highly branching, isomeric structures makes chromatographic separation with typical reversed-phase HPLC columns generally difficult. Moreover, oligosaccharide structures are usually in great diversity and the comprehensive…

    Words: 2206 - Pages: 9
  • Nothapodytes Nimmoniana J Graham Case Study

    dry leaf, fresh barks of plant, induced callus, somatic embryo and suspension culture (In exogenous medium and in pallet) in 100 mg/ml were analyzed for CPT content by TLC (Thin Layer Chromatography) and HPLC (High Performance Liquid Chromatography). TLC and HPLC analysis confirm the presence of CPT in dry leaf, fresh barks of the plant, induced callus and somatic embryo. Out of which bark extracts possessed a maximum amount of…

    Words: 731 - Pages: 3
  • Potato Dextrose Agar Essay

    fraction, 4-5 drops of Hager’s reagent (saturated solution of picric acid) was added and observed for the presence of yellow precipitate formation. Vinblastine and methanol was used as positive and negative control respectively. 4.5 Thin Layer chromatography…

    Words: 2097 - Pages: 9
  • Dissolved Gas Analysis Essay

    Calculations for Dissolved Gas Analysis in Water Samples Using a GC Headspace Equilibration Technique, EPA National Risk Management Research Laboratory, RSKSOP-175, Rev.2, May 2004. 1.2 Light Hydrocarbons in Aqueous Samples via Headspace and Gas Chromatography with Flame Ionization Detection (GC/FID), Pennsylvania Department of Environmental Protection Bureau of Laboratories, PA-DEP 3686, Rev.0, April 2012. 1.3 Lange’s Handbook of Chemistry, 14th edition (1992), McGraw Hill. 1.4 Gas…

    Words: 1252 - Pages: 5
  • Mitochondrial Function Lab Report

    2.7. Hippocampal mitochondrial function 2.7.1. Hippocampal mitochondrial isolation After MWM test was finished, all animals were sacrificed and hippocampal tissues were removed. These tissues were minced and homogenized with glass handheld homogenizer. Mitochondria were obtained from the rat's hippocampus by differential centrifugation [38]. In the first centrifugation (at 1500 g, 10 min at 4°C), all of the broken cell debris and nuclei were sedimented. Then the supernatant was centrifuged…

    Words: 1267 - Pages: 6
  • 2.2: Preparation Of Econazole Nitrate Nannoparticles

    2.2. Methods 2.2.1. Preparation of econazole nitrate nanoparticles The econazole nanoparticles were prepared by nano-spray drying technique. Weighed amounts of econazole nitrate, carriers and stabilizers (table 1) were dissolved in ethanol (10 ml) followed by spraying through the Buchi® nano-spray dryer B-90 (Büchi Labortechnik, Switzerland). The nano-spray dryer was operated using inert loop B-295 system (Büchi Labortechnik AG, Flawil, Switzerland) operated using nitrogen gas with flow rate of…

    Words: 1534 - Pages: 7
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