Purification And Characterization Of Biosurfactants Case Study

B Sridhar et al (2012) studied Purification and Characterization of Biosurfactants. The emulsifications of biosurfactants were checked in various oils such as olive, coconut, sunflower and rice bran oils, the emulsification activity of biosurfactants. Pseudomonas aeruginosa had higher emulsification activity than Saccharomyces cerevisiae. While checking the sugar and lipid content in biosurfactants by phenol sulphuric acid method and vanillin reagent method, found high in Pseudomonas aeruginosa than Saccharomyces cerevisiae. Under this study, after centrigugation of crude extract,the pellets were suspended in a 40 mL of dichloromethane in a separating funnel and shaken vigorously and the allowed surfactant recovered in the organic layer at …show more content…
Raquel Rufina et al (2012) have reported the Antimicrobial and Anti-Adhesive Potential of a Biosurfactants Produced by Candida Species. The antimicrobial activity of the biosurfactant isolated from Candida sphaerica was determined by measuring the growth inhibition percentages obtained for several Microorganisms. The tested biosurfactant presented antimicrobial activity against all microorganisms used, although, depending on the microorganism, the biosurfactant presents different effective concentrations. The highest concentration of biosurfactant tested (10 mg ml-1) showed high percentages of inhibition for Streptococcus oralis J22 (68%), C. albicans (57%) and Staphylococcus epidermidis (57.6%). The antimicrobial activity of the crude biosurfactant isolated from Candida sphaerica with concentrations between 5 and 10 mg ml1 against C. albicans, Staph. aureus and Staph. epidermidiswas less to that obtained with the biosurfactants. The crude biosurfactant showed antimicrobial activity against a broad range of microorganisms,including Gram-positive and Gram-negative bacteria and