Reverse Micellar Extraction Of L-Asparaginase Case Study

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1. Abstract
In this study, reverse micellar extraction of asparaginase model system was carried out using CTAB/Iso-octane/hexanol/butanol system to optimize the forward and back extraction efficiency. By using Taguchi’s orthogonal array (OA), maximum forward extraction efficiency of 55.0%, 61.0% and 54% was achieved with an aqueous phase pH of 11.0, 150mM CTAB/Iso-octane and 0.1 M NaCl, respectively. Similarly, Taguchi’s orthogonal array (OA) was applied to optimize the pH of stripping phase, concentration of Isopropyl Alcohol (IPA) and KCl for maximizing back extraction efficiency (BEE). The optimal levels of stripping phase pH, concentration of isopropyl alcohol (IPA) and potassium chloride (KCl) were found to be 6, 20%(v/v), and 0.8 M, respectively.
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Firstly, these processes are extremely expensive and are difficult to scale up. Further, the cost of the raw materials involved in purification are manifold. Downstream processing of asparaginase accounts to 70-80% of the total cost (Rati et al, 2014). An ideal purification process should reduce the downstream processing costs to a great extent and it must be time …show more content…
The cost of the raw materials involved in the extraction of the biomolecule from the crude is extremely economical. The RME system is greatly influenced by a number of parameters such as the pH, the type of salt used and its concentration, the type of solvent used, the type of surfactant used and its concentration, water content and volume ratio of the aqueous phase to the organic phase (Liu et al, 2008). The diffusion of biomolecules inside the micelles is governed by electrostatic, steric and hydrophobic interactions between the proteins and reverse micelles ((Hong et al, 2000), (Umesh et al, 2008)). Selective extraction of biomolecules in micelles involves two main steps. Firstly, the forward extraction in which the solubilization of protein from the aqueous solution to the organic phase of the reverse micelle takes place. In the second step, the protein from the reverse micelles is released into the aqueous phase for the recovery of the purified protein, known as back extraction (Yu et al,

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