Liquid chromatography-mass spectrometry

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    Keywords: Aralia continetalis Kitagawa; Kaurenoic acid; Phase I; Pharmacokinetics; Ultra-performance liquid chromatography-tandem mass spectrometry Abbreviations: AIC, akaike information criteria; AUC0-∞, the area under the concentration–time curve from zero to infinity; CL/F, the total body clearance for oral administration; Cmax, the maximum plasma concentration; CV, coefficient of variation; HPLC, high-performance liquid chromatography; ka, the absorption rate constant; IS, internal standard; LLE, liquid–liquid extraction; LLOQ, lower limit of quantification; MRM, multiple reaction monitoring; PK, pharmacokinetic; QC, quality control; t1/2, the terminal half time, Tmax, the time to reach Cmax, UPLC-MS/MS, ultra-performance liquid chromatography-tandem…

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    Introduction: One of the most common mass spectrometry–based proteomic methodologies used to day is the bottom up protein identification. First, the protein has to be digested into peptides using a protease. Then, the peptide will be submitted to the URMC Proteomics Resource and Laboratory for analysis using LC-MS/MS tandem mass spectrometry. In this approach, the peptides will be separated and introduced into a mass spectrometer using reversed phase liquid chromatography (RC-LC). This…

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    MDMA Synthesis

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    Solid-phase extraction is used to separate analytes from the sample matrix, as the analytes’ chemical and physical properties differ. Particularly cation exchange cartridges are widely used to determine methamphetamine and its metabolites. Since amphetamines are weak bases, their ionic form may bind to the sorbent. However, the mixture of organic solvent and alkaline solution, which is utilised for elution, must be removed before liquid chromatographic analysis. (Lee et al. 1997; Wood et al.…

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    Mass Spectrometry

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    Aim: Discuss the advantages, disadvantages and limitations of mass spectrometry for the analysis of both small molecules and proteins. Background Mass spectrometers. They are a powerful tool used to evaluate analytes such as single atoms, molecules, whole proteins, or the simple peptide chains that make them up. Using the mass to charge ratio (m/z) of these analytes, we can differentiate one or many entities (such as one particular peptide fragment, or a panel of them) from everything else in…

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    for 30 min at room temperature. Then, trypsin/chymotrypsin- digested samples of KPNB1 obtained from the UV-crosslinking experiments with various concentrations of compound 1 were analyzed by nanoflow liquid chromatography and tandem mass spectrometry. The tandem mass spectra of the trypsin/chymotrypsin-digested KPNB1-compound 1 adducts showed 10 peptides coupled to compound 1 (Table S1, the Supplementary Information). Among these adducts, we found that the amounts of three of the products,…

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    Separation of oligosaccharides appears essential to investigating complex oligosaccharide structures from plant origin. The chromatographic separation has been carried out in GC and HPLC using many types of stationary phase. The most commonly used technologies include hydrophilic interaction chromatography (HILIC), high performance anion exchange chromatography (HPAEC), and HPLC with porous graphitized carbon (PGC) column. Table 1.3 compares the advantages and disadvantages of some…

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    oligosaccharides will help in management of bioresources, production of industrially important biomolecules, reduction of environmental pollution but will also reveal promising therapeutic utilization of this by-product. Especially, structural elucidation of oligosaccharides from apple pomace enables the understanding and prediction of their biological functions. High resolution mass spectrometry and tandem mass spectrometry provides a powerful and highly sensitive analytical tool to improve…

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    transferring the mixed protein solution to isoelectric tube gel (pH 3-10) and subjecting it to a constant electric current (145mA). After the gel has been allowed to run for about an hour, the isoelectric tube will be rinsed with running buffer and set aside. Next, an SDS gel will be prepared in order to separate the proteins based on mass. This will be done by sandwiching two spacers between two glass plates and placing them within a plastic bag. The plate/bag assembly will then be placed into…

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    a Beckman DU650 spectrophotometer (Beckman Coulter, Fullerton, USA). Phenolic compounds were isolated using a multiple preparation HPLC system (LC-Forte/R, YMC, Kyoto, Japan) with a reverse phase preparative column (YMC-Actus Triart Prep C18, 20 mm × 250 mm, 10 μm). 1H and 13C NMR data were obtained usingn a Bruker AM 500 (1H NMR at 500 MHz, 13C NMR at 125 MHz) spectrometer (Bruker, Karlsruhe, Germany) using DMSO-d6 with TMS as the internal standard. For the quantification of the isolated…

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    Female Sprague

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    The liquid chromatography was performed using an Acquity UPLC® system (Waters Corp., Milford, MA, USA) coupled to a mass spectrometer (Xevo TQ-S, Waters Corp.). The bioanalytical method for the simultaneous quantitation of the three PFCs was optimized through the various mobile phase conditions and sample preparation processes. A 3 µL aliquot of each sample was injected into a Kinetex® 2.6 µm C8 100 Å column (2.1 mm × 100 mm, 2.7 µm particle size, Phenomenex, Inc., USA) with a column…

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