Extraction

the theory and practice of two laboratory techniques: solubility-dependent extraction, and recrystallization, in an effort to isolate and identify three compounds originally dissolved in a mixture. Extraction is used to isolate a specific compound. Recrystallization involves taking a crude compound and removing unwanted impurities so that a more accurate melting point can be taken, leading to a more precise identification of a compound. The overarching goal was to isolate and identify three different unknowns in a given sample using these techniques. There are two types of extractions; solid-liquid extractions involve extracting a soluble compound from an insoluble (solid) material…

The efficiency of each experiment was found by determining the percent yield of extraction, the percent yield of sublimation, and the total percent yield of caffeine. The percent yields calculated help determine the amount of caffeine recovered, which was the efficiency of this experiment.2 The first part of the experiment required the use of both solid-liquid and liquid-liquid separation in order to extract caffeine from the tea bags. The tea bad was first brewed and filtered. After brewing…

Extraction of Caffeine from Tealeaves Rosheen Villaraza, Dana Young, Dana Yu*, and Ray Zafra Department of Biological Studies, University of Santo Tomas, Manila, Philippines Abstract Experiment 3 highlights the separation of caffeine from tea leaves and using chromatography for testing its purity. Extraction is a method of separating the desired from the undesired substance in a mixture through the solubility properties of the substances. In the experimental extraction, Camellia sinensis was…

Embedded in tea granules is a compound that plays a role in daily life, Caffeine. In the food industry, pure caffeine can be used to create bolder coffee and rich flavorings. However, greater are the potential uses for this compound in medicine if it can be separated effectively from tea granules. Caffeine acts as a stimulant, making humans more mentally alert. Two methods used to separate caffeine from tea granules are Thin Layer Chromatography and High Performance Counter Current…

This lab was performed to both introduce and practice extraction techniques along with any procedures associated. The specific extraction employed used the different reactivates of the components with an acid and a base to produce products that are more soluble in water at different stages. Other methods included filtration of mixtures, obtaining a compound’s melting point, and using infrared spectroscopy to analyze a compounds structure. Since no initial masses were given, percent yield cannot…

Extraction involves dissolving a compound or compounds either from a solid into a solvent or from a solution into another solvent. In extractions it is important to mix the layers very well to get a clear visual of the two solvents in the solutions. We use the solvent extraction techniques to effectively separate an organic, Base, Acid and neutral compounds. Within each of these layers the experiment is modified to fit each of the different situations. So when looking at the solids, carboxylic…

The extraction lab began with a .517g mixture of benzoic acid, p-nitroaniline, and azobenzene charged in a 125 mL Erlenmeyer flask mixed with 50 mL of Et2O. Once dissolved, the mixture is transferred the mixture into separatory funnel and begin acid extraction. 15 mL of 3 M HCl is added to the separatory funnel and inverted until a phase boundary is present. An additional 15 mL of 3 M HCl was then added and inverted again. The aqueous layer was removed through the separatory funnel and placed…

Today in lab we worked/talked about DNA. DNA is molecules called nucleotides. What is the main purpose of DNA ? The main role of DNA in the cell is the long-term storage of information. Where is the DNA found in the body ? Most DNA is located in the cell nucleus. Okay so in lab, we did an experience. We used A Heavy duty ziploc bag, 1 strawberry [including the stem], 10 mL DNA extractions buffer [ soapy, salty water], Cheesecloth, Funnel, 50mL vial/ test tube, Glass rod, Inoculating loop, or…

Tooth extractions at times are a necessity in helping to preserve good oral health and it has long been practiced throughout history and can be traced back centuries. Dental extractions in the United States are done in a private office or in a hospital setting depending on the severity of the tooth and or teeth. Certain types of extractions can be done in a private office for example if you have a fully erupted wisdom tooth you can have it extracted in a private office, but if you have an…

Alveolar Ridge Resorption: Alveolar bone resorption is a normal physiologic response following teeth extraction. The causes of alveolar bone volume loss can occur before dental extraction due to periodontal disease, periapical pathology and trauma to the teeth and bone. (SchroppL et al.2003) (1, 2). Additionally, the traumatic removal of teeth can cause bone loss and must be prevented (1,3). Finally, the alveolar bone suffers atrophy after tooth extraction, which has been well documented (1, 4,…