environmental testing, they look for PCB’s in oil, and pesticides in ground water. In the food industry, they can test additives to determine concentrations of preservatives, vitamins, proteins, and amino acids.2 Chromatography is the process of separating a mixture, and has many different applications throughout the world. There are a variety of ways to conduct chromatography including column chromatography, paper chromatography, and thin layer chromatography. The particular experiment conducted and discussed is paper chromatography. This experiment will use chromatography to test and identify unknown pen inks based on tests done on known pen inks.
Understanding the history of chromatography can help us understand different uses of the technique. A Russian botanist invented chromatography to separate and distinguish plant pigment. Mikhail Semenovich Tsvett trickled a mixture of dissolved pigments through a glass tube filled with a tight calcium carbonate…
Paper Chromatography Lab Discussion
The results of the Paper Chromatography process seem to have aligned with what was expected. When the capillary action on the paper had completed and the chromatograph was finished, the distance that each cation moved (D), as well as the distance that the solvent moved (L), could be properly measured. The distance that the solvent moved ended up being 7.05cm, a number that would represent the constant variable L in…
What is it?
Gas chromatography is a variation of chromatography used in analytical chemistry for separating and analysing components that can be vaporised without decomposition ("Gas Chromatography", 2016). This technique involves using a gas as the mobile phase. There are two types of gas chromatography:
• Gas-solid chromatography – separates substances on the basis of their different strengths of adsorption on the solid
• Gas-liquid chromatography – separates…
Chromatographic process is a separation technique which has to do with the mass transfer of test sample between a stationary and mobile phase. HPLC (High Performace Liqud Chromatography) is a vast system which involves the use of a mobile and stationary phase to separate materials. The stationary phase is usually a column packed with solids (usually silica gel) while the mobile phase is usually a solvent or a mixture of various solvents. The mobile phase usually, is the carrier of…
5.05 LAB REPORT
Paper chromatography is a widely used method of separation. The lab will show the basic techniques of paper chromatography. In this lab, the separation of the dyes used in two different kinds of candy is performed. The candy dyes may also be compared to solutions of FD&C food dyes.
The purpose of this experiment is to…
(1997) also isolated ferritin via protein expression of pET3 vector that contains 5F12 coding sequence in the NdeI sequence site for the H-type subunit of the frog ferritin. The vector host was Escherichia coli BL21-3 cells grown in medium containing sorbital and betaine. Ferritin synthesis was induced in mid-log cells using isothiopropyl thiogalactoside (IPTG), and the soluble ferritin was isolated, after sonication of the bacterial cells, by fast protein liquid chromatography (FPLC) on a Fast…
Affinity Chromatography, also referred to as biospecific chromatography, is a technique of partitioning rooted in biological affinity (Dean). This practice capitalizes on complex interactions such as bond formation between enzymes and substrate, or more broadly: macromolecules and ligands. Purification is at the center of the use of affinity chromatography. In general, samples may be run through a gel that contains a corresponding component that binds with the sample at certain…
Chromatography is a separation method used to help identify mixtures. One common form of chromatography is gas chromatography. This type of chromatography will usually use a gas as the mobile phase, and a liquid as the stationary phase (“Gas Chromatography”). A gas chromatograph is comprised of some vital components, which include: the carrier gas, flow controller, injector port, column, column oven, detector, and recorder (“Gas Chromatography”). The purpose of this lab was for an unknown…
just 10^2. LBAs can detect both, active and free forms of a large molecule drug in samples. Aside from this aspect, they take a long development time of months and have low specificity may be because of its antibody cross reactivity. With all these limitations of LBAs, they can not detect diverse proteins and peptides required for biopharmaceutical industries.2 LC-MS on the other hand provides an intact analysis, with th information of a whole peptide molecule.
LC-MS Bioanalysis :
HPLC column can be categorized into two types
• Reverse Phase
• Normal Phase
18.104.22.168. Reverse Phase Chromatography
Reverse Phase system has a non-polar stationary phase over which polar solvents are used as mobile phase; it is generally used for separation of polar species.
22.214.171.124. Normal phase chromatography
Normal Phase system has a polar stationary phase over which non-polar solvents are used as mobile phase; it is generally used for separation of non-polar species.. A detection…