The role of paper chromatography is to separate various and different chemicals/mixtures to show individual components within. If there are any individual components within the mixture, then they will move up the paper at different speeds and this will make the components separate.
When carrying out the process, drawing an origin line near the bottom of one of the ends on the chromatography paper will be the starting point of the mixtures that you are trying to separate when they move up the paper. At first hand, the paper will be soaked with a solvent to allow a stationary liquid phase (this phase is when the mixture being tested is being carried through). The components of the mixture may be soluble in different ways so choosing a running solvent that is suitable for this will allow the components to be separated out. The running solvent …show more content…
This is where a locating agent is used, the agent is joined with the chemical in the spots. In some cases, further chemicals is used and added to allow a reaction with the locating agent to finally create a coloured spot.
Since the general structure of an amino acid includes an amino and carboxyl group, the R group of the amino acids separates all the different types and making each one of it unique. In the R groups, aromatic rings, COO- groups, -NH3+ groups, sulfur containing residues etc, are examples of the different structural features that can be found. The amino acids will have a mixed variety of this features, so therefore they will not be all the same and be individually on its own.
The pure being of the R groups also determines the different properties of one amino acid. These properties can include its physical state, how soluble it is and its own mechanical strength.
Highlighting the solubility of the amino acids will determine its movement up the chromatogram because it is dependent on it along with the running
When carrying out the process, drawing an origin line near the bottom of one of the ends on the chromatography paper will be the starting point of the mixtures that you are trying to separate when they move up the paper. At first hand, the paper will be soaked with a solvent to allow a stationary liquid phase (this phase is when the mixture being tested is being carried through). The components of the mixture may be soluble in different ways so choosing a running solvent that is suitable for this will allow the components to be separated out. The running solvent …show more content…
This is where a locating agent is used, the agent is joined with the chemical in the spots. In some cases, further chemicals is used and added to allow a reaction with the locating agent to finally create a coloured spot.
Since the general structure of an amino acid includes an amino and carboxyl group, the R group of the amino acids separates all the different types and making each one of it unique. In the R groups, aromatic rings, COO- groups, -NH3+ groups, sulfur containing residues etc, are examples of the different structural features that can be found. The amino acids will have a mixed variety of this features, so therefore they will not be all the same and be individually on its own.
The pure being of the R groups also determines the different properties of one amino acid. These properties can include its physical state, how soluble it is and its own mechanical strength.
Highlighting the solubility of the amino acids will determine its movement up the chromatogram because it is dependent on it along with the running