Paper chromatography

Cheyenne Wilson 5.05 LAB REPORT CANDY CHROMATOGRAPHY BACKGROUND INFORMATION: Paper chromatography is a widely used method of separation. The lab will show the basic techniques of paper chromatography. In this lab, the separation of the dyes used in two different kinds of candy is performed. The candy dyes may also be compared to solutions of FD&C food dyes. Purpose: The purpose of this experiment is to…

Introduction Affinity Chromatography, also referred to as biospecific chromatography, is a technique of partitioning rooted in biological affinity (Dean). This practice capitalizes on complex interactions such as bond formation between enzymes and substrate, or more broadly: macromolecules and ligands. Purification is at the center of the use of affinity chromatography. In general, samples may be run through a gel that contains a corresponding component that binds with the sample at certain…

using isopropyl alcohol (C3H8O) in paper chromatography. Scientific Explanation When leaves are mixed with isopropyl alcohol (C3H8O) the alcohol draws out the leaf pigments and chemically change to a solvent. When the solvent is painted on the paper towel nothing happens but when a bit of alcohol is added it easily climbs up the paper towel. This happens because the alcohol molecules are “sticky” and when they combine with something adhesive such as a paper towel they will want to connect.…

separate chlorophyll a and chlorophyll b and the alpha and beta conformations using the paper and column chromatography. The two techniques that were used to separate and identify the plant pigments were the paper chromatography and column chromatography. For the mixture to be separated, it must first be applied to a stationary phase. The stationary phase in the paper chromatography experiment was the filter paper. The mobile phase used in this experiment to help separate the pigments were the…

Chromatography is a separation method used to help identify mixtures. One common form of chromatography is gas chromatography. This type of chromatography will usually use a gas as the mobile phase, and a liquid as the stationary phase (“Gas Chromatography”). A gas chromatograph is comprised of some vital components, which include: the carrier gas, flow controller, injector port, column, column oven, detector, and recorder (“Gas Chromatography”). The purpose of this lab was for an unknown…

Introduction: The main purpose of this experiment was to take the Gas Chromatography/ Mass Spectrum part of the “Analysis of US bills for cocaine using gas chromatography / mass spectrometry” lab and improve upon the gas chromatographic results. This would be done by finding a way to separate the peaks of cocaine and doxepin. The lab done previously showed the use of two different methods to obtain samples of cocaine and doxepin for spiked dollar bills. The two extraction methods were…

Raju Chandra et al; A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of quetiapine fumarate from marketed bulk tablets. The active ingredient of quetiapine fumarate separation achieved with C18 column using the methanol water mobile phase in the ratio of 30:70 (v/v). The active ingredient of the drug content quantify with UV detector at 359 nm. The retention time of quetiapine fumarate is 5.27 min. A good…

4.3 High Performance Liquid Chromatography (HPLC) An HPLC analysis was carried out for the Fraction C2-7 for confirmation of the results obtained by TLC analysis. According to the HPLC chromatogram, there is only one peak was observed at the retention time of 4.510 minutes. Therefore, it can be concluded that there is only one compound presents in the Fraction C2-7. Basically, the first few small peaks in the chromatogram which is around 2-3 minutes are ‘noise’ from the injection that been made…

potential designer modification of banned drugs and show the same elemental composition and yielding identical nominal, exact masses and general analytical properties which make the potential of misidentification increase. Electron- ionization gas chromatography- mass spectrometry (EI-GC-MS) technique was used as initial methods to differentiate and separate the target compounds. In addition, consecutive confirmation methods was provided by…

MDMA (3,4-methylenedioxymethamphetamine), commonly referred to as ecstasy, and methamphetamine are central nervous system stimulants, which can produce euphoria, enhanced mental and emotional clarity, sensations of lightness and floating, and other hallucinations. (Chandra et al. 2015) Due to their effects, MDMA and methamphetamine are used in Drug-Facilitated Crimes (DFC), intended to impair behaviour, perceptions, or decision-making capacity to take advantage of an impaired person without…