Paper chromatography

The mixture was stirred, and a white precipitate of dicyclohexyl urea (DCU) was created after 15–30 min. The reaction was monitored by thin layer chromatography (TLC). After approximately 6–8 h, the precipitate was filtered and the filtrate was evaporated to dryness. The resulting residue was dissolved in acetone, filtered to additional remove any DCU, and evaporated once more. 10% sodium bicarbonate…

Table 1 Composition of Lansoprazole microparticles Formulation code Amount of drug(mg) Amount of polymer chitosan (mg) Drug polymer Ratio Tween 80 (%w/v) F1 100 100 1:1 1.25 F2 100 200 1:2 1.25 F3 100 300 1:3 1.25 F4 100 400 1:4 1.25 F5 100 500 1:5 1.25 F6 100 600 1:6 1.25 Characterization of Lansoprazole microparticle The microparticle will be characterized for following parameters: Percentage yield: After complete drying, the drug loaded microparticle was collected and weighed accurately…

distilled water. The vacuum filtration is used to dry the curds for five minutes. After this step, the lab groups were to combine the wash from the vacuum filtration with the first filtrate. Once this step is done, place the curds on several layers paper towels and blot…

Objective The objective of the experiment was to determine the purity and components of a mixture of fluorescein and bromonitroindoline, as well as separate them, through the process of thin layer chromatography. Procedure Part A- Separation of Compounds by Thin Layer Chromatography • Three 100 mL beakers and watch glasses were cleaned in preparation for this experiment. Three silica gel plates were acquired from the instructor, and to each plate a line was drawn 5 mm from the edge of the…

Anion exchange chromatography: In anion exchange chromatography negatively charged ions will bind to the column. This will leave positively charged ions flushed through the column. By picking a pH of 6.5, protein X will flush through the column while protein Y and Z will stick to the sides of the column. Then by changing the pH to 8 it would make protein Z negatively charged and will be stuck in the column as protein Y is flushed to the bottom of the column. Cation exchange chromatography: This…

amount of benzoic acid recovered could have been caused by a number of possibilities. Some of the components may have been lost throughout the extraction process, some of the component may have stuck to the inside of the reaction tube or the filter paper used in vacuum extraction, or may have been human error in recording the masses. In all of these occurrences may have contributed to the recovery loss of benzoic acid. Conclusions Overall, all three components benzoic acid,…

Tempkin isotherm contains a factor that explicitly takes into account adsorbing species-adsorbate interactions . This isotherm assumes that the heat of adsorption of all molecules in the layer decreases linearly with coverage due to adsorbate-adsorbate interaction and adsorption was characterized by a uniform distribution of binding energies, up to some maximum binding energy. Tempkin isotherm has generally been used in the linearized and rearranged form as following:…

pH meter: Weighing balance. Materials: HPLC grade Methanol, HPLC grade analytical water, Acetonitrile, Membrane filter paper- 0.45µm was used. Selection of Detection wavelength: Individual dilutions of Dapagliflozin and Saxagliptin were prepared from standard stock solutions using methanol. Both the solutions were scanned over the range of 200-400nm on UV-VIS spectrophotometer…

Twenty Korean barley cultivars (Geongang, Gwangan, Nackyoung, Dahyang, Daejin, Saegang, Boanchal, Keunal, Saessal, Saechalssal, Sujung, and Boanchalssal) were selected in this research. All the cultivars were developed by the National Institute of Crop Science (NICS), Rural Development Administration (RDA), Korea and were grown during 2013 in the experimental fields of the NICS, Milyang, Gyeongnam. After harvesting, barley leaves were immediately washed with clean sterile water and then…

successful separation of the pigments from the spinach leaves, the pigments are put into silica gel and the solvent is allowed to evaporate off so that the pigments (now in powder form) can be put through column chromatography to separate the chlorophyll and β-carotene pigments. Column chromatography is a means to divide chemical compounds from a mixture. Separation is achieved by eluting solvents through the column which hold similar properties to one or the other pigment. Chlorophyll, being a…