Paper chromatography

Adsorption is the bond of particles, particles, or atoms from a gas, fluid, broken down and attached strongly to a surface. This procedure makes a film of the adsorbate on the surface of the adsorbent. This methodology varies from absorbtion, in which a liquid (the absorbate) pervades or is absorbed by a porous solid. Adsorption is a surface-based procedure while absorption includes the entire volume of the material. The term sorption envelops both methodologies, while desorption is the converse…

Experiment 5 Synthesis and NMR Spectroscopy of ethyl cinnamate Introduction: Wittig reactions involve the stereoselective synthesis of olefins from phosphonium ylides and aldehydes or ketones, where the carbonyl bond is converted to an alkene double bond.1 The variety of suitable reagents and the relatively mild experimental conditions facilitate their industrial applications, such as the synthesis of epoxides, esters, carotenoids and vitamin A.2,3 Specifically, the ester ethyl cinnamate is used…

Slime is made by mixing together borax, glue, and water. Depending on the amount of borax added to the mixture will determine the density of the slime. For our experiment in class the independent variable was the borax. The dependent variable was slime. The control group was glue. It was important to keep the amount of glue, borax, and water constant throughout the experiment. In an experiment conducted in class it was hypothesized that the more borax added the denser the slime will be.…

Every substance reacts with force. Whether it breaks, splashes, or holds together. The science behind this experiment is the suspension. The suspension is the mixture of two substances. Where one is finely divided and dispersed in the other(SteveSpanglerScience,2015). The purpose of this experiment is to analyze the different states of viscosity, varying on the force applies to the solution. This mixture has two different states when force is being applied. When touched slowly, it is simple to…

Thin Layer chromatography was also use to separate the mixture of fluorene and fluorenone. Thin layer chromatography is a useful separation method when the components of a mixture are in question. Analyzing what the mixture consist of can be efficiently determine by TLC by calculating Rf and comparing these values. For the separation and isolation of a mixtures components, this method is extensive and not as efficient at column chromatography. This conclusion is represented in the data gathered.…

Observe Ligand-Protein Interaction using Gel Filtration Chromatography (Phenol Red Binding to Bovine Serum Albumin) Introduction: Serum albumin is often known as blood albumin, and is the most abundant protein in humans and other animal plasma. Serum albumin is also known as a carrier protein by non-specifically binding several biomolecules. It is essential for maintaining the osmotic pressure that is needed for body fluids distribution between body tissues and intravascular compartments.…

meso-tetraphenylporphyrin (100 mg, 0.162 mmol) dissolved in dichloromethane (100 ml, 1.57 mol) in a water-jacketed flask was irradiated with compact fluorescent lamps. The solution was then concentrated in vacuo and the product subjected to column chromatography to yield the product, aldehyde, and unreacted starting butadiene which could then be recycled. 1-Phenylbutadiene…

Chromatography is a process used to separate molecules on the basis of chemical or physical properties, such as molecular mass, charge, or hydrophobicity. (Ninfa 121) There are many types of chromatography processes that can be used in different experiments and scenarios. These chromatography processes on include Gel filtration, ion exchange, and high pressure liquid chromatography. However, in this laboratory session the process of affinity chromatography is utilized to purify a His-tagged…

Introduction: Acetaminophen is a common pain reliever and fever reducer used to treat conditions such as head, muscle or tooth ache, as well as colds, fevers, and even arthritis. It is available in a tablet form to be ingested. These tablets are manufactured by a large variety of pharmaceutical manufactures including a great deal of generic brand companies. These tablets all have the same active ingredient, acetaminophen, yet each hold different inactive ingredients that can impact the overall…

materials to find the chromatography of Skittles Sweets and Sours and Crayola markers. Using a ruler and a pencil, a line was drawn on all thirty chromatography strips 1.5 centimeters from the bottom of the paper. This was to ensure that the starting point for each chromatography process was consistent. The chromatography of the skittles was found first. We began by pouring water into a cup. A pipette was used to transfer fifteen single drops of water from the cup onto a paper plate. The…