Paper chromatography

Introduction Thin-layer chromatography, otherwise referred to as TLC, is a fast and inexpensive way to determine the amount of components in a solution or to identify an unknown compound. The principal behind TLC is when different compounds are placed on silica gel or alumina, (stationary phases) they will travel different distances when eluted with the proper solvent. According to ChemistryGuide, this is because the more polar compound has a stronger interaction with the silica gel due to its…

the alleged sticky bandit. The note was sent to the Tempe CSI Crime Lab for further analysis. Blaine Benson, head of the Tempe Crime Lab, has reported that paper chromatography will be used to identify the pen the bandit used. Pens from local syrup competitors were collected as evidence to compare to the ink used in the note. Paper chromatography is a common practice used to separate the colored components in the ink. The Rf value or the retention factor is the ratio of the distance traveled by…

3.3.9. Encapsulation efficiency of drug loaded NPs The encapsulation efficiency (EE) was determined by weighing the amount of unloaded drug recovered from lyophilization of the supernatant obtained after centrifugation of NPs. The drug encapsulation efficiency was calculated according to the following equation: Encapsulation efficiency (%) = (Winitial/Wfree)/Winitial *100%. Where, Winitial and Wfree are the weight of initial drug and the weight of free drug determined in the supernatant after…

To assess the bioequivalence by comparing the single oral dose bioavailability of test product MYCOCEPT 500mg (containing Mycophenolate mofetil 500 mg) film coated tablets manufactured by (confidential) with that of reference product CELLCEPT 500mg (containining Mycophenolate mofetil 500 mg) film coated tablets distributed by Roche Registration Limited, shire park, welwyn Garden City, AL7 1TW, UK in healthy, adult, human subjects under fasting conditions. 4.2. STUDY DESIGN A randomized,…

RESULT AND DISCUSSION 7.1. Preformulation studies 7.1.1. Identification of pure drug by FTIR Figure7.1: Identification of Sample through FTIR Figure 7.2: FTIR Spectrum of Standard Prazosin hydrochloride (with references of B.P.- 2009) Table: 7.1 - Characteristics peaks of Prazosin hydrochloride S.No. Reference peaks (cm-1) Theoretical peak of Prazosin hydrochloride Obtained peaks (cm-1) Functional Group Stretching/ Bending 1 1750-1680 1529.92 1529.92 C=O Stretching 2. 1650-1580…

1. Abstract In this study, reverse micellar extraction of asparaginase model system was carried out using CTAB/Iso-octane/hexanol/butanol system to optimize the forward and back extraction efficiency. By using Taguchi’s orthogonal array (OA), maximum forward extraction efficiency of 55.0%, 61.0% and 54% was achieved with an aqueous phase pH of 11.0, 150mM CTAB/Iso-octane and 0.1 M NaCl, respectively. Similarly, Taguchi’s orthogonal array (OA) was applied to optimize the pH of stripping phase,…

Schiff Bases: Schiff bases are amongst the important class of drugs containing imine or azoethine (-C=N-) moiety,have been obtained by the condensation of primary amines and carbonyl compounds.It was first time presented by the Hugo schiff (Cimerman et al, 2000). Schiff bases are one of the most important class of organic drugs widely used in variety of different fields such as analytical, biological, and inorganic chemistry.The schiff bases heve been designed to synthesize more and more novel…

The purpose of this experiment was to identify the generic name of the unknown drug through thin-layer chromatography analysis. By comparing the Rf values obtained from each TLC plate, it was deduced which components were present in the unknown drug. Through comparison of Table 1 and Table 2, Unknown A was identified as B.C. Tablets. Salicylamide was evident in the unknown substance due to the distinct blue color that was present in both the unknown spot and the salicylamide spot; the similar Rf…

During this experiment that was conducted in partners, the concentration of RNase H that was purified in the previous experiment #6 was determined and also the concentration of an unknown given solution using their absorbance values obtained from the spectroscopy apparatus. These concentrations were calculated using the equation of the line produce with the values of the concentration (x-axis) and corresponding absorbance (y-axis) of five protein bovine gamma globulin standard solutions. In…

Introduction 1.1 Polyurethane polyurethanes were first developed by Otto Bayer and coworkers in 1937. Shortly thereafter, Schollenberger and coworkers used formula modification to prepare elastomeric polyurethane products from Bayer's products that were like nylon or other engineering plastics in physical characteristics. PUs are widely used in coatings, flexible and rigid foams, elastomers, and composites. Reasons for the use of polyurethane coatings include: high performance characteristics…