High Performance Liquid Chromatography Lab Abstract
High performance liquid chromatography or HPLC is the process in which mixtures are physically separated according to their polarity. The lab conducted, the separation of dyes in grape Kool-Aid, demonstrated the concept. This was done to display how different alcohols and their concentrations have different polarities, in which ties into HPLC. The lab consists of testing Kool-Aid, water, and different concentrations of methanol and isopropanol; 5%, 20%, and 60%. To start, one must pour 10 ml of the Kool-Aid into a syringe that was connected to a C-18 absorbent. The plunger that came with the syringe will then be pushed through into a labeled test tube designated for the substance. Once
High performance liquid chromatography or HPLC is the process in which mixtures are physically separated according to their polarity. The lab conducted, the separation of dyes in grape Kool-Aid, demonstrated the concept. This was done to display how different alcohols and their concentrations have different polarities, in which ties into HPLC. The lab consists of testing Kool-Aid, water, and different concentrations of methanol and isopropanol; 5%, 20%, and 60%. To start, one must pour 10 ml of the Kool-Aid into a syringe that was connected to a C-18 absorbent. The plunger that came with the syringe will then be pushed through into a labeled test tube designated for the substance. Once