Chromatography is a process used to separate molecules on the basis of chemical or physical properties, such as molecular mass, charge, or hydrophobicity. (Ninfa 121) There are many types of chromatography processes that can be used in different experiments and scenarios. These chromatography processes on include Gel filtration, ion exchange, and high pressure liquid chromatography. However, in this laboratory session the process of affinity chromatography is utilized to purify a His-tagged protein RNase H. The Separation of molecules that happen during affinity chromatography is based on specific interactions between an enzyme and an substrate. These interactions, which are typically reversible, are used for purification by placing one of
