RNA polymerase

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    RNA Polymerases

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    “However, the DNA-dependent RNA polymerase proved elusive until 1960, when it was independently identified in bacteria by Hurwitz and Stevens and in plants by the Bonner group” (p. 8325). There were also many experiments after these in which the scientists focused mostly on the mechanisms of the RNA polymerization (Kireeva, 2013). Kireeva also stated in her article that a group called the Chamberlin group focused on the pauses by E. coli RNA polymerase the group also focused on the…

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    Brains were sectioned at 16 µm in a cryostat, mounted on Super-Frost Plus slides with embedding medium and stored at -80℃. Signals of ISH were detected by digoxigenin (DIG) labeled single strand cRNA probes. The DIG-labeled cRNA probes were transcribed in vitro from cDNAs of rat GR (~0.4 kb) and rat SERT (~0.7 kb) in pGEM-3Zf vectors with T7 RNA polymerase and DIG RNA labeling mix (Roche, Branford, CT, USA). The amount of the transcript was estimated by comparison to DIG-labeled control RNA…

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    A. What is the purpose of a polymerase chain reaction (PCR)? List the four main components of a PCR. (2 points) The purpose of a PCR is to make large amounts of DNA segments from just a specific DNA sequence. This method is used to scientifically prove if a suspect of committing a crime is truly the person who committed the crime. The four main components of a PCR are: DNA from a sample, nucleotides A, T, G, C, DNA polymerase, and primers. B. What is gene expression? List the two steps of…

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    Protein Synthesis Paper

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    This paper is going to describe the replication of DNA and RNA and the processes of transcription and translation of protein synthesis. What is DNA? DNA is a nucleic acid that carries the genetic information in cells and some viruses, consisting of two long chains of nucleotides twisted into a double helix and joined by hydrogen bonds between the complementary bases adenine and thymine or cytosine and guanine. DNA sequences are replicated by the cell prior to cell division and may include genes,…

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    Ebola Virus

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    joint pain, liver inflammation, and decreased hearing capabilities among other difficulties. Those who survive can no longer transmit the disease. Negative sense, single stranded RNA (ssRNA) constitutes the virus’s genome. It is considered to be a negative sense because the RNA cannot be directly translated into functional proteins. It must first be transcribed into mRNA, which is complementary to the ssRNA, before it can be translated. The ssRNA is contained in virions that are cylindrical or…

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    eukaryotes is that genetic information is formed in protein and is converted into organization, function and cellular structure. Eukaryotes also have a replication of machinery that is found in bacteria but are more complex and larger. The phase of replication has more factors for helicase and primase complexes, it then loads onto a sliding clamp unit where polymerase is there as…

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    Eukaryotic Synthesis

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    Transcription is considered to be the first step of gene expression. It is known to be the process of making a copy of genetic information stored in a DNA strand into a complementary strand of messenger RNA (mRNA). The process of eukaryotic transcription can be separated into three phases, initiation, elongation and termination. This complex process involves various cell signaling methods as well as the activity of many enzymes. Genes are segments of DNA nucleotides located on a chromosome…

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    3.05 Dna Research Paper

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    RNA polymerase attaches itself to a template of DNA and then go into base pairing, synthesizes mRNA or messenger RNA. This is called transcription, as the DNA code being transcribed into mRNA code. RNA replaces Thymine for Uracil during base pairing. 4. mRNA leaves the nucleus and enters the cytoplasm this goo like part of the cell where ribosomes can be found. 5. Ribosomes are involved in protein synthesis, in other words they create proteins. 6. mRNA attaches one end to a ribosome, and through…

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    Protein Molecules Essay

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    Proteins Molecules Building materials for cells. Cells have organelles such as mitochondria, lysosomes, vesicles, etc. It is estimated that the body has over ten trillions cells Carry oxygen, build tissue, copy DNA for the next generation and do the work needed by the body. Deoxyribonucleic acid contains the blueprint for proteins and replication of itself mRNA transcribes DNA, tRNA translates mRNA into proteins. A pattern on nucleotide on a DNA strand is called a gene. The Central Dogma of…

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    Lujo Virus Essay

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    a. Introduction Lujo Hemorrhagic Fever is caused by a bi-segmented negative RNA virus that is one of several viruses known to cause viral hemorrhagic fever. The Lujo virus is part of the Arenaviridae family, which consist of several viruses categorized into two groups. The Lujo virus is categorized in the “Old World” group, which signifies that the virus in question was found in the Eastern Hemisphere, specifically the continent of Africa. The majority of the arenaviruses categorized in the…

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