GraphPad Prism

The data from the cytotoxicity study was analyzed using factorial analysis of variance (ANOVA) with statistical software Dell Statistica (data analysis software system) version 12, Dell Inc, Tulsa, USA, country (2015) available from http://www.Software.dell.com. The data from total GSH and ROS assays were analyzed using “one-way ANOVA followed by Dunnett’s multiple comparison tests with GraphPad Prism version 6.00 for Windows (GraphPad Software, La Jolla California USA, http://www.graphpad.com.anova using Graphpad software). The effect of binary mixture of metals (Cd + As, Cd + Pb and As + Pb) and B[a]P + metals (B[a]P + As, B[a]P + Cd and B[a]P + Pb) on cytotoxicity in HepG2 cells are presented in Table 4 and Figures 1 to 6. Arsenic (As), Cadmium (Cd) or lead (Pb) either alone (main effect) or binary combinations, Cd + As, Cd + Pb and As + Pb (interaction effect) caused significant cytotoxicity to HepG2 cells (Table 1, Fig. 1…

All the powders were protected from light by wrapping the vials with an aluminium foil. After one month storage, the powder samples were evaluated for drug content, in vitro aerosolization, surface morphology and solid state characterization by X-ray diffraction studies following the above mentioned procedures. Statistical analysis All the data was processed and their mean ± standard deviation values were determined using the Microsoft® Excel spreadsheets. Statistical analysis of the data (mean…

(1:200 dilution, Roche, Branford, CT, USA) was applied to sections at 4oC overnight. The color was developed by NBT/BCIP substrates (Roche, Branford, CT, USA ) with refreshments twice a day for 3~5 days. The images of in situ hybridization were quantitatively analyzed with Image J software (Rasband, US National Institutes of Health, Bethesda, http://rsbweb.nih.gov/ij, 2010) by setting the thresholds and analyzing the particles. The values of area fraction shown in the summary results indicated…

minutes. In order to determine the proportion of PI-positive cells, the nuclei were labeled with propidium iodide (PI) and the cover marked with Neutral Balsam. All negative control sections were incubated in PBS without primary antibodies to further confirm the specificity of the antibodies. Five random fields of each animal were selected and the images were visualized by the use of a fluorescence microscope (Olympus BX51, NIKON, Japan). The Statistical Analysis The data in this report were…

tissue rings and were fixed in 10% neutral buffered formalin solution. Then, the samples were paraffin embedded, cut into thin sections, and mounted on microscopic slides. The slides were stained by a Modified Movat 's Pentachrome staining protocol. Three representative images, each of which covered approximately 1/3 of the media layer, were captured and used to obtain the average contents of the tissue components in this layer. Aortic aneurysms are attributed to medial degeneration [25]; hence,…

amount of ATP and ADP were measured by the standard curve and then the ratio was calculated. 2.8. Hippocampal mercury measurement Mercury concentration was measured in brain hippocampus by cold vapor atomic absorption spectrometry (CVAAS) (Semi-automated Mercury Analyzer, model Hg-201, Sanso Seisakusho Co. Ltd., Tokyo, Japan). Briefly, a homogenized sample (0.5 g of wet weight) was digested with a mixture of 2 mL of nitric acid-perchloric acid 1 + 1 (HNO3-HClO4) and 5 mL of sulfuric acid…

respectively. However, at nine months of on-going stability point the microbiological count raised at a relatively higher rate with TVAC of 1475 CFU/ml. The breaking point of the generation time for the bacteria was expected to be between 3-6 mo with an slow initial rate from 0-3 mo and fast one from 6-9 mo. Since no count was detected till the point of 6 mo, the generation time was estimated to be between 1.89 to 41.28 mo (56.67 to 1238.49 d) with the average middle point (5 CFU/ml) to be 6.28…

temperature for 10 min, the absorbance of the mixture was read at 562nm against a deionized water blank using an Ultraviolet-Visible (UV-vis) spectrophotometer PERSEE TU-1810 UV-VIS (Persee Instruments co. Ltd, Beijing-China). EDTA was used as the positive control. The percentage of the inhibition of ferrozine-Fe2+ complex, expressed chelating activity was calculated using the following equation: Chelation (%)=100(1-As/Ac) Where As is absorbance of sample or EDTA, and Ac is absorbance of…

washes with buffer. 6- A detection antibody will be added to each of the wells for 30 minutes incubation at room temperature. 7- Streptavidin-phycoerythrin conjugate will be added to each of the wells and washed 3 times with wash buffer. 8- By using Bioplex 200 instrument the plate will be scanned at both a low PMT (normal) and high PMT, and statistics computed using suitable software and analyses. 4- Statistical Analysis: Biostatistical analysis will be performed using all available…

gMFI value as 0%. The percentage of activation of THP-1 cells exposed to various concentrations of H. pylori product (in the presence of LPS) was calculated by comparing the CD80 gMFI values of the samples to the gMFI values of negative and positive controls (Fig. 4B). The values (y axis) were then graphed against product concentration (x axis) (Fig. 4C) and a 4 parameter logistic (4PL) curve-fit was performed (Fig 4D). % activation= (CD80 gMFI (Sampl〖e)-CD80 gMFI (negative control)〗_ )/(CD80…