Flow cytometry results, Figure 3, demonstrate that the initial population of MDA-MB-231 cells contain the sub-population of cells with the desired surface markers, CD44+/CD24-/low/CD45-. The flow cytometry data showed only ~4% of the total cells represent the sub-population with the desired surface markers. In a study using flow cytometry to identify stem-like cells, not including CD45 in their experimental analysis of MDA-MB-231 cells and similar cell types demonstrate that 90% of the population express the phenotype CD44+/CD24-/low phenotype (Fillmore & Kuperwasser, 2008). Other studies have found similar results looking at the same phenotype. In the current CD45 was added to the selection for stem-like cells and to flow cytometry assays.…
culture medium. The first THP-1 cell culture was made by Dr Senta Walton, with a starting concentration of 2 x 105 cells/mL. The cell culture was then incubated at 37°C with 5% CO2 and subcultured every 2-3 days, when the cell concentration reached approximately 8 x 105 cells/mL.
THP-1 cell based assay
THP-1 cell based assay was performed to determine the immune modulatory effects of H. pylori and its derived products. In this assay, THP-1 cells were stimulated by 0.5 µg/mL (0.1 µg/well)…
1ml per sample. The samples were incubated at 4⁰C overnight. The following day, the samples were washed with DPBS at 4⁰C and then washed with 2ml 1 perm buffer twice. Anti-mouse Foxp3- PE Ab was added in a 1:100 dilution for 30 mins at 4⁰C. The samples were washed with perm buffer and resuspend in DPBS for flow cytometry analysis.
Table 2.4 Antibody panel for SHPS-1 Phenotyping
Panel PerCP FITC APC PE PB
DC 1 CD45 CD11b CD11c CD103 B220
DC 2 CD4 CD11b CD11c CD8 B220
Treg CD3 CD25 FoxP3 CD4
I have a love/hate relationship with Pinterest. I signed up after being egged on by my two sisters who both enjoyed collecting pins at the time. I've lost track of my younger sister's activity-being a social butterfly she's most likely moved on to bigger and better things, but my older sister persisted-especially after the arrival of her first child. I pinned at first with enthusiasm, but the pressure to complete pins I'd added to my boards began to haunt me, and a few feeble recipe attempts…
While participating in a Sun Salutation Practice being run in Brisbane, Australia, Henry had a chance to chat with Alana who was running the practice. We talked about how hard it was for both student and teacher to count the full 108 rounds of the practice session. Since you were having to keep count of the rounds, this did not allow you to enter the flow state of mind. Instead, you had to constantly work to remember where you were in the count.
Is There an App for this?…
1. I have completed my survey for the Information Technology Infrastructure Library Course and have received my passing score 26\40.
2. Kim Rymsha’s computer is back in her office and both her personal and network printers have been connected to her Pc.
3. Docsvault has been completely installed on Lisa Cabrera.
4. I have unlocked Angel Li, of Fiscal, 10th Floor Active Directory Account.
5. I am awaiting the status for pickup of our DBH Inventory of old Equipment that has been updated for…
A BAD BET
• Does God Oppose Gambling? • Is Gambling Sin? • What Does the Bible Say? Dr. Russell K. Tardo According to St. Augustine, “The devil invented gambling.” Whether or not one accepts Augustine’s conviction, gambling has been around for thousands of years. Augustus Caesar is said to have sponsored the first known public lottery in order to raise funds to repair the city of Rome. The Bible records that Roman soldiers gambled for the garments of Christ (Matthew 27:35), an action…
1 Feb. 2013
Videogames and Violence
In today’s society, the vast majority of adolescents spend their free time indoors. Many of those hours are enjoyed playing video games, a habit which teenagers will never hear the end of from their parents. “These games will ruin you”, they say, “It makes you angry and violent”, they say, but does it really? Media often portrays videogames as detrimental to mental health, and this topic is especially scrutinized today because of the…
Personal Cash Flow Statement Assignment
How does a personal cash flow statement help you organize your finances?
A personal cash flow statement gives you the ability to separate your finances in diverse groups so you can receive a idea of where certain parts of your income is going, I enjoy the ability to see details about my finances. Like when you go shopping the cashier gives a receipt, this allows you to see how many items you purchased. Spending plays a large part in your cash flow…
Case 30: Wynn Resorts, Ltd.
Business Policy Development and Implementation MGT 451
October 20, 2012
Case 30: Wynn Resorts, Ltd.
Where does the Wynn on the Cortai Strip fit on the Wynn Resorts Ltd. Growth and Expansion strategy? Is there support to make a significant capital investment in this resort? Why or why not?
The Wynn on the Cortai Strip fits well within the Wynn Resort’s growth and expansion strategy due to the significant size of land for expansion…