Cell culture

Cell culture & cell counting: Introduction: Cell culture is the method of removal of cells from their respective animal or plant source, followed by their growth in a favourable artificial environment. These cells can be removed directly from tissues or fragmented using enzymatic or mechanical method. These cells can be obtained from the already established cell line or cell strain. Short protocol:In this protocol, initially cells were washed to remove adherent cells, incubated and supernatant was removed. Pellet was examined, resuspended in the fresh medium and incubated in the cell culture medium. For counting cells were washed to remove adherent cell, centrifuged, resuspended in required medium and counted using haemocytometer.…

shaped modern science tremendously. Fields such as cell biology, molecular biology, biochemistry and many others are heavily dependent on cell culturing to carry out experiments. Cell culturing initially began when pioneering scientists sought a way to study cells outside their tissue origin. They began with tissues and then later on, techniques were developed that enabled the study of individual cells in vitro that would give rise to animal cell culturing. In this summary of cell culturing,…

Methods and materials Cell culture medium Making cell culture medium, requires two processes, this includes the medium that has PSG and other without the PSG. 15 ml of 10% FBS was added into a culture flask with 1.5 ml 100x hippos. The culture flask was filled with DMEM up to 150 ml and this will be the medium without the PSG and this process completed the medium without the PSG. The same procedure was followed for the PSG medium, 1.5 ml of PSG was added and that completed the medium with PSG.…

Cells stolen from Henrietta Lacks, a black woman who died of cervical cancer during the first half of the twentieth century, led to dozens of groundbreaking medical discoveries. Despite this contribution, her family lives utterly destitute, her name forgotten by all but her most dedicated followers. Rebecca Skloot’s book attempts to correct this injustice, giving life to the woman many simply know as HeLa. Skloot’s The Immortal Life of Henrietta Lacks hammers the point home by using pathos to…

HeLa cells were the basis of cell culture in the latter half of the 20th century. In The Immortal Life of Henrietta Lacks, Rebecca Skloot explores the scientific achievements and ethical issues relating to HeLa cells, as well as the connection HeLa cells have with the Lacks family. When Henrietta Lacks was being treated for cervical cancer, the doctors shaved parts of her tumor off and sent them to a lab, where her cancerous cells never stopped dividing. She never consented to have her cells…

who did cell culture research as a means of gaining fame and fortune at the expense of others. This is simply not true, as Dr.Gey was a truly selfless man, whose only goal was to expand the horizons of what cell culture research could bring for the better good of the human race regardless of how much money or fame he got. Dr.Gey was never a man with a silver spoon in his mouth. “According to Rebecca Skloot author of “The Immortal Life of Henrietta Lacks” when Gey was attending the…

to the fact that ethics was not yet a crucial role in science. Scientists have been experimenting on Henrietta’s cells (HeLa) cells for decades, and even now the cells are being used in labs. Since the HeLa cells divide indefinitely, scientists can study and analyze them without running out of supplies. Over the years, these cells have greatly contributed to science, but more specifically, the vaccine for polio. Polio is an infectious disease caused by a virus that claimed thousands of lives…

Eppendorf tube. Transfer supernatant to 1.5 ml tube and snap frozen in liquid nitrogen then kept at -80 °C until hormonal analysis. Trypsinization were used for harvesting live cell, 500 µl of pre-warmed trypsin-EDTA were added to each well and incubated at 37.5 °C for 5 min. Then dislodged cells from well by pipetting and to stop trypsin effect, 100 µl of heated inactivated fatal calve serum (HI FCS) were added to each well and mixed well. Transfer cell suspension into 1.5 ml Eppendorf tube,…

Budget Justification Personnel Vijaya M. Lella (also known as L. Vijaya Mohan Rao), Ph.D. Principal Investigator, 3.6 calendar months effort and salary. The P.I. will devote 30% of his effort on the proposed grant. He will assume overall responsibility for the proposed project, which includes supervision of all personnel involved in the project, planning, the design of experimental approaches, the close review of the data, data analysis & interpretation, preparation of manuscripts, and decisions…

“Henrietta Lack’s cells thrived. In weight, they now far surpassed the person of their origin and there would probably be more than sufficient to populate a village of Henriettas” (237). According to the quote above, the amount of cells from one woman’s body is compared to the population of one village. How could this be? Rebecca Skloot, the author of The Immortal Life of Henrietta Lacks, first started learning about HeLa cells in school, but was more curious to know the origin of the cells.…