Enzyme

Introduction An enzyme is a protein that functions as a catalyst, it speeds up chemical reactions (Freeman et al. 2017 p. 90). Enzymes are often large globular proteins and are able to hold substrates in specific orientations so they are able to react, the location where the substrate binds with the enzyme and reacts is called the active site and is the location where the catalysis occurs (Freeman et al. 2017). Enzymes activity is often related to the optimal environment for them in respect to…

influence of high pressure on the kinetics of α-chymotrypsin The choice was determined by the fact that the catalytic mechanism as well as pressure stability of this enzyme is well established. α-chymotrypsin-catalyzed hydrolysis proceed according to the Michaelis–Menten mechanism. The initial process involves creation of a stable enzyme substrate complex ES, which is related to KM, afterwards activation of the ES^≠ and subsequent reaction steps occur, which are governed by the catalytic rate…

leaning more towards 55ºC. As we can tell from the results, temperature does have an effect on enzyme activity. As predicted, amylase activity increased until it reached its optimum temperature, where it was at its peak in terms of how effective it was in breaking down starch. At relatively high temperatures (in comparison to the optimal temperatures of the bacterial and fungal amylases), the enzyme denatured and was unable to hydrolyze the starch present in the solution. The enzyme’s…

As a result, lactose is a competitive inhibitor. In the presence of the inhibitor, the slope of the Lineweaver-Burk plot becomes the equation below. Using equation 7 along with the Km and the Vmax from series A, the Ki of Series B and C were calculated. The concentration of inhibitor for 50mM lactose was found to be 35714 μM using equation 1. Similar to the substrate concentration, the inhibitor concentration can be found using the same equation, but substituting in the inhibitor molar…

of the liver lab was to understand how enzymes, more specifically catalase, break down poisonous chemicals, hydrogen peroxide. In the lab we used multiple reactants, temperatures, and pH levels. For the tissues and enzymes we used livers, new, used, cold, warm and boiled, chicken breasts, potatoes, and apples. We also used hydrogen peroxide, warm and cold, acidic and basic, and used liquid from a reaction. Hydrogen peroxide acts as a substrate for the enzymes. The reactants were the catalyst of…

the conclusion that the more enzymes available, the larger and faster the reaction. The data obtained is in agreement with the hypothesis. The hypothesis stated that the rate of reaction and amount of product produced will augment with the increased concentration of the catalase. It was observed that in the 80% catalase concentration the most amount of O2 was produced, while in the 10% catalase concentration the least amount of O2 was produced. The higher ratio of enzyme to substrate…

form AMP, releasing the energy for two groups of phosphate of course at the end of the process of the molecule. The two cellular reactions that involves ATP hydrolysis includes the following; firstly, we have a reaction called enzyme-catalyzed reaction and this case enzyme works as a catalyst for protein and the second reaction is the one that takes place during cellular respiration and that is the organic fuel oxidation. 2. What do ribosomes do? What is their relationship to…

effect does temperature have on the pepsin when breaking down tofu Introduction: This practical looking for the change that temperature has on the enzyme activity of pepsin on tofu. Enzymes act as catalysts and help quicken the process of breaking down foods that are called substrates, in digestive systems(Rader, A., 2016). In this experiment the enzyme is pepsin and it breaks down proteins into peptides, it is a common in the digestive system and helps breakdown the proteins that are in…

was to observe the enzyme catalysis of Horseradish Peroxidase with different determining factors. In this experiment the horseradish peroxidase enzyme should have an optimal temperature of 35°C, should have an optimal pH of 6, and should catalyze rapidly with the substrate and enzyme concentration. We were able to observe that the enzyme reacts best with its optimal temperature of 35° C and optimal pH of 6. Moreover, when there’s a high the substrate concentration and a high enzyme concentration…

In a biochemical reaction, enzyme which are proteins based compounds are used as a catalyst to produce a high yields. Proteins are the macromolecular components of almost enzymes, except for ribozymes, a class of RNA modifying catalysts. Enzymes are classified on the basis of their composition; simple enzymes are those composed wholly of proteins, whereas, complex enzymes which also known as haloenzymes, composed of protein plus a relatively small organic molecule. In this terminology the…