Enzyme inhibitor

Introduction of Angiotensin-Converting Enzyme Inhibitors LeMone & Burke (2011) stated that in other to accurately discuss angiotensin converting enzyme inhibitors, it is very important to first of all briefly discuss about the three forms of angiotensin. Angiotensin I is produced by the action of renin on a protein called angiotensinogen, which is formed by the liver. Angiotensin I is then transformed into angiotensin II in the blood by the action of angiotensin-converting enzyme ACE. Angiotensin II therefore causes contraction of the muscle surrounding the blood vessels that leads to the narrowing of the vessels. When this process occurred (narrowing of the vessels) it causes the pressure within the vessels to increase which leads to hypertension, elevation of blood pressure.…

Change the amount of enzyme Table 4: The absorbances of 5 cuvettes that measured at 475nm during a given time interval using spectrophotometer. Time (s) Absorbance @ 475nm Cuvette 1 Cuvette 2 Cuvette 3 Cuvette 4 Cuvette 5 0 0.003 0.003 0.004 0.002 0.009 30 0.003 0.004 0.008 0.013 0.021 60 0.005 0.005 0.013 0.016 0.031 90 0.005 0.008 0.019 0.020 0.040 120 0.007 0.011 0.022 0.033 0.051 Figure 4: The graph of time vs. absorbance of 5 cuvettes that contained different amount of…

insufficient communication is a barrier that inhibits collaboration in online courses. This aligns with research that suggest that the asynchronous nature of many online courses creates an environment that includes a waiting period that, in some cases, allows time for the student(s) to go ahead and find the answer or solution before the instructor or peers have an adequate time to respond (Monahan, McArdle, & Bertolotto, 2008). However, 41.1% of participants disagreed or strongly disagreed that…

Introduction Enzymes are an important biological catalyst that is absolutely crucial for life to exist. The ultimate purpose of this experiment was to examine enzymes and how they function in cells, explore the result that concentrations and environmental factors have over these functions, and lastly to read and interpret graphs related to enzyme function. This type of study had various parts, therefore there are various hypotheses. The first being, if it is potato extract, then it will contain…

Result and discussion: Substrate specificity The substrate specificity for the PPO is represented in table. The Km values for catechol, L-DOPA, L-tyrosine, and p-Cresol are 4.12 × 10-3, 16.4 × 10-3, 6.12 × 10-3, and 5.01 × 10-3, respectively. Catechol was the best suited substrate, having the lowest Km value and highest Vmax value when compared to other 3 substrates (Table 1). Table 1: Substrate specificity of PPO enzyme Substrates Specific activity (units mg-1 protein) Km (µM) Vmax (abs min-1)…

of Competitive Inhibitors on Catechol Oxidase Enzyme Activity. Jaley Willingham, 2015, Functional Biology, Texas State University, San Marcos, 78666. Abstract Competitive inhibitors may have an effect on enzyme activity. We investigated the effects that parahydroxybenzoic acid has on the enzyme activity of catechol oxidase. For this experiment, different levels of parahydroxybenzoic acid concentration were added to catechol oxidase. Each level of concentration was then tested for the absorbance…

1 enzyme Enzymes are biological catalysts. A protein compound Energy can reduce the carbon reaction. Enzymes to accelerate specific types of reactions. And the kind of substance that reacts. 2.1.1 catalysis of enzymes E is a catalyst (enzyme). S is a precursor called chop, nitrates and substance P is a product. E + S ---------------> E S ---------------> E + P. Complexometry 2.1.2 Factors affecting the enzyme. 1) What type of controlled…

Enzymes are known as catalysts that cause reactions to occur more rapidly by lowering the reaction’s activation energy. A reaction’s activation energy is known as the minimum amount of energy required to get a chemical reaction started1. In order for cells to efficiently manage thousands of different chemical reactions, they make use of biological catalysts. Without these catalysts, biological reactions would not be able to proceed at a proper rate necessary to maintain life. Most, but not all,…

As a result, lactose is a competitive inhibitor. In the presence of the inhibitor, the slope of the Lineweaver-Burk plot becomes the equation below. Using equation 7 along with the Km and the Vmax from series A, the Ki of Series B and C were calculated. The concentration of inhibitor for 50mM lactose was found to be 35714 μM using equation 1. Similar to the substrate concentration, the inhibitor concentration can be found using the same equation, but substituting in the inhibitor molar…

Enzyme catalysis can be a very confusing and long process, but in easier terms can just be explained by the fact that enzymes break down molecules into smaller pieces. The objective of this lab is to observe how quickly the enzyme catalase breaks down H2O2, and what long periods of contact between the two do to that process. We used seven different cups for seven different lengths of time, and in every cup we mixed 10 mL of 1.5% H2O2, 1 mL of distilled water, and 1 mL of catalase. We then…