Enzyme inhibitor

Acid in the stomach functions to kill bacteria, and aid digestion by solubilizing food. The acid is also important to establish the optimal pH (between 1.8 and 3.5) for the function of the digestive enzyme pepsin. Stimulation of acid secretion involves the translocation of H+/K+-ATPases to the apical membrane of the parietal cell. When the cell is resting (not stimulated), H+/K+-ATPases are located in vesicles inside the cell. When the cell is stimulated…

experiment, the effect of environmental conditions on enzyme functions was tested. The materials needed for the experiment was an amylase enzyme to lower the activation energy of the reactions, starch solutions of pH 3,4,5,6,7,and 8, these provided different concentrations to project various environmental conditions. In addition, Iodine solution, and a spectrophotometer were needed. Each cuvette contained a specific pH concentration. Then the enzyme extract was added to each pH concentration…

Enzymes are biological highly selective catalyst, greatly accelerating both the rate and specificity of metabolic chemical reactions, from the digestion of food to the synthesis of DNA. Enzymes catalyze chemical reactions involving the substrate. Substrate is a molecule that attach to the active site of the enzyme protein which an enzyme acts. Active site is a small port in an enzyme that helps enzyme to bind with substrates and undergo a chemical reaction. During this binding of substrates with…

The purpose of conducting this experiment was to explore how different factors affect the reaction rate of enzymes reacting with their corresponding substrates in order to learn more about how enzymes function in different environments. The independent variables investigated in this experiment were the concentration of different substrates, the temperature of the environment, and the effect of a catalyst on the reaction rate. The dependent variable for all of the investigations was the time it…

DISCUSSION Nelson et al, 2008, state “all enzymes that exhibit a hyperbolic dependence of V0 on [S] are said to follow Michaelis-Menten kinetics”. The data gathered from this experiment and plotted in figure 6 shows that it is this pattern shown in the reaction of PNPP to PNP catalysed by acid phosphatase, where a hyperbolic curve is observed in the graph of rate of reaction against substrate concentration, approaching the Vmax asymptotically. Acid phosphatase behaved congruently to the…

Km and Vmax determination of glucocerebrosidase towards p-NPG The Michaelis constant (Km) is a measure of the affinity of the enzyme towards the substrate, with smaller values representing greater affinity. Km and the maximum rate (Vmax) of leukocyte GCase were obtained through the Lineweaver-Burk plot (Figure 4) with artificial substrate p-NPG in concentrations from 0.71 mM to2.50 mM. Km and Vmax values for leukocyte GCase using p-NPG as substrate was found to be 12.6 mM and 333 U/mg…

The catalytic effect and specificity of enzymes 1.Introductions to enzymes Enzymes are proteins that can react with their specific substrates to achieve an highly effective catalyst reaction. .Almost all cellular activities of enzymes involved in the process are required to improve efficiency. Similar to other non-biological catalysts，enzymes provides another reaction route requires lower activation energy (Ea) to increase the number of particles that have a kinetic energy of not less than…

Introduction: A human has thousands and thousands of different proteins, each with a specific structure and function. Along with their diverse functions, they vary in structure, each protein having its own unique three-dimensional shape. Proteins are polymers of amino acids joined together by peptide bonds. There are 20 different amino acids to make up all the different proteins on the earth. Each of the amino acids is composed of a central carbon bonded to a hydrogen, a carboxyl group, an amino…

Enzymes are biological catalysts that speed up the various chemical reactions that happen inside our cells. For the enzymes to function well, they need to be in an environment with a specific set of conditions, other than that it will lead to the denaturation of the enzyme or will render it completely inactive. There are a lot of factors that impact enzyme activities, but in this experiment we examined the effects of 4 different factors which are temperature, Ph level, addition of the inhibitor…

For the first part, the qualitative polyphenol oxidase test (Table 1), we looked at 3 qualitative test tubes, where only test tube 2 turned orange since this tube had both the 40 mM dopa, the substrate and PPO enzyme extract. However, the test tube 3 did have a cloudy component because of the presence of PPO extract, but there was no color change. For a color change to occur, there needs to be a presence of both the dopa and PPO extract. Furthermore, we calculated the final concentrations of…