Amylase

Abstract: Amylase in the body is used to break down polysaccharides into monosaccharides. The monosaccharides are simple sugars that are used to give the body energy. This effect was investigated in an experiment designed to show the breaking down of starch solution. A water bath was used to heat water up to 60oc. Pieces of dialysis tubing were wet and had starch solution put inside. Half of the measuring cylinders were filled with 60oc water, and the other half with 25oc water. Tests of the water were carried out with an iodine test and diet stix to detect any starch that had been broken down and passed through the tubing. The results from the tests show that there were experimental errors, and the experiment should be carried out again to…

various background experiments and studies, such the human evolution theory which suggests that primates gained sufficient nutrients from starch not meat, it states that starch may have played a vital role in human evolution and how humans have more copies of amylase now, which further perceives that amylase may have adapted to changes in evolution overtime. Amylase a vital enzyme, and it plays many significant roles within the body. Professor Alicia, date (…....) discussed that the lower levels…

the activity of the human amylase is 37°C. This suggest that enzymes have to adapt to the temperature of the organisms in order to develop work because the normal temperature of human is 37°C. It is important to understand that the enzyme amylase play a role to breakdown, so other enzymes can produce sugar of it. In humans, it is critical to develop work at human’s temperature to continue developing break down the molecules. For example, it is important in humans, so it can break down all the…

In this lab experiment, we set out to test the efficacy of the enzyme Amylase in different pHs and heat. The objective of the experiment was to prove that the enzyme Amylase is the least efficient in a higher or more basic pH. According to the data, the hot solution of amylase, most likely due to the UV rays. The wavelength of the hot solution was 0.6696nm, significantly smaller than the other solutions. The base, which was predicted to be the least effective came in at 2nd least effective at…

in this experiment was salivary amylase. Salivary amylase is an enzyme that helps with the breakdown of carbohydrates, specifically starch (Tracey et al. 2016) and works in the mouth (VanPutte et al. 2014). Salivary amylase works to hydrolyze the – 1, 4 –glycosidic linkages between the maltose and glucose monosaccharides in starch (Tracey et al. 2016).…

50oC and 70oC) have an effect on the reaction rate of enzyme amylase and starch? Hypothesis As the temperature increases, the enzyme will have a quicker reaction time with the starch, therefore when met with the iodine, no change will occur in the colour of the solution, showing that the starch is now absent. The enzyme amylase and starch solution in 4oC and 25oC will change the colour of the iodine to a dark blue-black in the first few minutes, showing that the reaction hasn’t been completed,…

previous step but add the letter S to signify starch solution. For the test tubes labeled S, add 5 mL of starch solution. To continue to the effect of temperature on amylase activity, add 1 mL of amylase into each of the…

Analysis and Discussion The hypothesis that amylase would no longer hydrolyse the starch solution into maltose past 37°C was not supported by the data. Fig 1b, Fig 2.1 and Fig 2.2 all show the colour samples for various temperatures taken at increments of 10 seconds up to 40 seconds. Initially, 4°C was the slowest followed by 19°C then 37°C, 45°C, 80°C and 60°C being the fastest acting in the first 30 seconds. 80°C was the second-fastest acting temperature up until 20 seconds, but slowed down…

In this experiment, the effect of environmental conditions on enzyme functions was tested. The materials needed for the experiment was an amylase enzyme to lower the activation energy of the reactions, starch solutions of pH 3,4,5,6,7,and 8, these provided different concentrations to project various environmental conditions. In addition, Iodine solution, and a spectrophotometer were needed. Each cuvette contained a specific pH concentration. Then the enzyme extract was added to each pH…

Biuret test. 6) There was a positive digestion in this tube because we added PH 7 and albumin solution to it and were able to function because the PH was within the range of enzyme digestion. Part C • The enzyme used in this experiment is Pancreatic Amylase for starch. It digests starch into reducing sugar. Hydrolysis occurs in duodenum and the PH ranges are from 7.1 to 8.2. • In this experiment, we used Starch solution, Lugol’s regent, and Benedict’s reagent. We used Lugol’s reagent as an…