does catalase affect the initial rate of formation and total amount of O2?
In water, hydrogen peroxide converts naturally into diatomic oxygen gas and water through the collision of water molecules. However, this reaction occurs at an extremely slow rate. With the presence of an enzyme, catalase, the reaction occurs at a much faster rate. This is because catalase binds with the hydrogen peroxide substrate at its transition state to weaken the covalent bonds, reducing the energy and precision needed for a collision of water molecules to yield a reaction.
In our cells, there are numerous naturally occurring reactions which produce hydrogen peroxide. However, hydrogen peroxide is a toxin to the human body.…
Catalase is an iron porphyrin enzyme present in high concentrations in humans and animals liver tissues. It is a peroxisomal enzyme that is responsible for destruction of toxic H2O2 released during certain metabolic processes.The activity of catalase can be determined by measuring the decrease in H2O2 concentration which is the substrate.
An enzyme is a large protein that acts as a biological catalyst which changes the rate of a reaction. It provides an active site…
Concentration and Temperature on a Catalase Reaction Rate
Catalase is a vital component in the human body. The most crucial of which would be Enzymes, these are protein catalysts. A catalyst speeds up a chemical reaction rate. Virtually all chemical reactions within the human body require a catalase enzyme to be completed in the proper amount of time. The decomposition of hydrogen peroxide to oxygen and water can be completed with the catalase enzyme (Thompson, 2005). In the…
Temperature and Substrate Concentration on Catalase
Biology 101 Lab Section 041
Catalase is an enzyme found in every living organism and is essential for cells. This lab explores how catalase reacts to four different temperatures and how the concentration percentage of substrate affects its reaction rate. The performance of catalase is dependent on many factors, so the purpose of the lab was to find the ideal concentration and temperature for…
catalysis breaks down hydrogen peroxide into water and oxygen. In this lab we conducted a series of experiment to find the effect of concentration of substrate and the temperature of catalase enzyme.
Introduction: There are a lot of factors that can affect the rate of enzyme reaction such as the concentration of the substrate, temperature of the enzyme, and shape of the enzyme. In this experiment we only measure how the concentration of the substrate and temperature of the enzyme affects the…
Effect of Temperature and Substrate Concentration on Catalase Reactions
Biol 101 Lab
February 10, 2016
The human body contains thousands of tiny structures called enzymes that perform specific functions. One enzyme called “Catalase” is often used to regulate the amount of a toxic compound, hydrogen peroxide, in the body. The purpose of this experiment was find the different factors that can affect Catalase-Peroxide reactions. The first part of this experiment…
The effect of temperature on the rate of reaction on Enzyme reaction
This experiment will investigate the effect of temperature on the rate of reaction of the enzyme catalase. The biological catalyst called enzymes increase the rate of reaction without themselves being chemically changed. Enzymes are globular proteins folded into a complex 3-dimensional shape that contains a special surface area called the active site where a specific substrate binds structurally and chemically.…
tract, cause sepsis, fever, appendicitis, abscesses, arthritis, pneumonitis, and endocarditis and has been known to be fatal because it is resistant to most antibiotics. It has been seen in the respiratory tract of cystic fibrosis patients which is known to be pulmonary disease (Kaláb, 2011). This organism is can be found in the alimentary tract as a harmless saprophyte Most of the time this bacteria is seen in people who ae immunosuppressed because it is an opportunistic pathogen. According to…
The oxygen becomes the gas and the water becomes the foam. Unlike the cow liver that was dropped into water, it did not react. The enzyme catalase was not activated to break anything down thus having no change on the cow liver.
Based on the evidence stated above, the hypothesis is supported because the hydrogen peroxide ended up breaking down the cow liver emitting gas and foam. There was no reaction with H2O because no enzyme was activated.
Effect on the…
hydrogen peroxide. these two test tubes are in a 250 mL beaker containing approximately 125 mL of tap water and ice. The thermometer was placed in test tube 7, which contains the hydrogen peroxide to measure the temperature of the solutions. The hydrogen peroxide was poured into the test tube containing to the liver and was observed.
Figure 2: Diagram of the apparatus used
The independent variable is what is being measured in the experiment.
The temperature of the…