Catalase Lab

The results obtained from the experiment and as illustrated above clearly show that an increase in the volume of O2 produced is accompanied by an increase of catalase concentration; though the lower concentrations suffer from slight inconsistencies. This leads to the conclusion that the more enzymes available, the larger and faster the reaction. The data obtained is in agreement with the hypothesis. The hypothesis stated that the rate of reaction and amount of product produced will augment with the increased concentration of the catalase. It was observed that in the 80% catalase concentration the most amount of O2 was produced, while in the 10% catalase concentration the least amount of O2 was produced. The higher ratio of enzyme to substrate …show more content…
Across the catalase concentrations, their O2 productions during the first minute were fairly alike. The 80% concentration produced a fairly large amount of O2 and continued to produce O2 at a fairly steady pace. In the lower concentration, particularly the 10%, it is observed that while it started producing fair amounts of O2, its production soon waned. The experiment showed that with the passing of time, the production of the different catalase concentrations diverges. An explanation for this observation may be that because the enzyme was not fully dissolved into the substrate, the catalase settled on the bottom of the test tube over the course of the 5 minutes. This reduces the surface area of the catalase available; this also reduces the amount of reaction able to take place at one particular time. The higher catalase concentrations were able to counteract the settling of the catalase, because their higher catalase to substrate ratio meanwhile the lower concentration had a disadvantage because their catalase concentration didn’t contain enough catalase molecules sustain the rate of production with which they had started …show more content…
The catalase enzyme is found in many organisms; however for this particular experiment purred banana was used. The product of this was the chunky liquid used as the catalase. The consistency of the banana puree was not one that lent itself to easy handling and because of this, measurements of the catalase, particularly the minuscule ones, were prone to be less accurate. For the most accurate measurement possible it would be advised to use a more suitable food item with characteristics that lend themselves to blending, such as perhaps potatoes or carrots. Failing this however a syringe with a wide opening tip would be preferable to a graduated cylinder and would provide a more precise