Catalase

Most proteins are enzymes, which have an essential role in biological catalysis by increasing the rate of a reaction. The experiment conducted included an enzyme assay with the enzyme catalase and the substrate hydrogen peroxide. To complete the assay, the catalase enzyme was added to the hydrogen peroxide buffered solution. Every thirty seconds, portions from the tube was removed and placed into the labeled tubes. Based on the degree in color of each sample, a different absorption value would…

1. The primary reaction catalyzed by catalase is the decomposition of hydrogen peroxide to form water and oxygen, which occurs spontaneously, but not at a very rapid rate. Write a balanced equation for this reaction. Label the reactant and the product. (Remember that catalase is not a reactant or a product and can be written over the arrow separating the reactant from the products.) I will help you with the equation. Catalase 2H2O2 ------------------------> 2H2O + O2 (g) (Hydrogen…

educated conclusion. After performing the gram staining and observing which unknown culture was gram positive, a catalase test was performed. The catalase test allows lab workers to differentiate Staphylococci and Streptococci genus. The test was performed only on the gram-positive culture. Bacteria that respire by the use of oxygen, otherwise known as aerobic respiration, produce enzyme or “catalase”. This protects the bacteria from the harmful by-product produced from oxygen metabolism that…

aided in conformation of these two samples, which was the MSA fermentation plates, the beta hemolysis of the blood and DNA agar plate, the coagulase test, Antimicrobial substibility, the characteristics that were viewed under the microscope, and the catalase test. Out of these test, the determining factor in detecting positive or negative confirmation for Staphylococcus aureus is through the coagulase test which is further explained in Table 1 and figure 5, the results were positive (Kateete).…

Catalase is an enzyme that accelerates the decomposition of hydrogen peroxide into oxygen and water. Catalase is a tetramer, composed of four monomers wrapped into a dumbbell shape (Nguyen). Each monomer has a heme molecule, a part indispensable to the decomposition of hydrogen peroxide. The heme molecule…

DATA ANALYSIS The linear relationships between the time elapsed and the volume of gas indicates a constant enzyme reaction rate. The activity rate of catalase in figure 1 is calculated to be approximately 0.15mL/sec. The following figures show how this rate of reaction is affected by different external factors. The results in figures 2 and 5 show an increase in either enzyme concentration or substrate concentration will increase the enzyme activity. In figure 2, the 0.75% enzyme concentration…

From the above tables and graphs, the results indicate that the optimum pH level of Catalase is at the neutral pH of 7 with an increase in enzyme activity from pH 2 to pH 7 and a decrease of activity from pH 7 to pH 12. Hence, demonstrating a bell shaped curve (similar to theoretical data – See Background Information for reference) which can be seen as an effect of the specific pH on the catalase enzyme. This identified trend can also be supported by the raw data values of the control test tubes…

Lau 15 September, 2015 Enzyme Catalysis I. Purpose Measure the activity of catalase in potato extract using several different methods. II. Procedure a) Cut 20 small circles from a large filter paper disk using a hole puncher. Clean and dry several medicine cups and pour hydrogen peroxide into one. Put the catalase enzyme, previously prepared by blending a potato, into another medicine cup. Make a 1/8 dilution of the catalase by adding chilled distilled water. Use tweezers to grab one of the…

bacteria from other bacterias the following medium was used: MSA plate, SIM plate, nitrate reduction test Nutrient gelatin and Urease broth. For Metabolic tests, only Catalase test were performed on the unknown bacteria. These tests were best use gram negative bacteria to indicated what the unknown microbe is. For the Catalase test, the use of hydrogen preoxide on the sample of the unknown…

The second test tube 11 was identified as a gram negative bacteria. The biochemical tests that I performed that were positive were catalase, glucose, SIM, indole, citrate, and urea. First, the catalase test has the presence of an enzyme in the test isolated detected using hydrogen peroxide. Bacteria possess catalase with a small number of bacteria; isolation is added to hydrogen peroxide when bubbles occur. When the bubbles occurred on gram positive bacterium it was a conformation of being…