Catalase

Horseradish peroxidase (found in the roots of horseradish) is a member of a large family of enzymes known as peroxidases; hemoproteins that can catalyze one-electron oxidation of a substrate in the presence of peroxide. It is largely an alpha-helical protein which binds heme as a redox cofactor. The “heme nitrovinyl” group is specifically where nitrite modification of the heme macrocyle occurs in HRP, in the presence or absence of hydrogen peroxide. It’s regioselective at the 4-vinyl group…

Hemoglobin has a peroxidase-like activity, meaning that it will break down hydrogen peroxide, or other organic hydroperoxides. This makes it useful in forensics, since you can test for the presence of hemoglobin simply by dropping hydrogen peroxide on it. Of course, there are a lot of things that have peroxidases in them, so this is a presumptive test for the presence of blood. Blood cells are not immortal; each cell wears out in about four months, and will disintegrate in the blood stream,…

In this essay, I will explain how we can benefit from bonding of atoms of certain elements in our daily life. As I will show in this essay, with the help of understanding the surface chemistry, which is the study of chemical reactions at interfaces (surfaces), different desired affects can be produced by the formation of selected elements such as making improved chemical composition of a surface. Surface chemistry today can be applied to solve various issues and problems including fighting…

Enzymes are catalysts that contain proteins. Catalysts are the part of the product that speeds up a reaction. Only reactions can occur when the right enzyme ends up finds the right material to produce a process. A low pH is a good environment for an enzyme as well as a low temperature. After the reaction, no enzymes are used up. They can be used again and again in reactions, once an enzyme is used in one reaction, it can be used in another. Enzymes are made up of proteins and proteins are made…

6. Quantitative determination of rat peroxisome proliferators activators γ (PPAR-γ): Rat PPAR-γ concentration was determined by ELISA; using a commercially available kit, MyBioSource, Inc., California, USA. The kit utilizing a monoclonal anti- PPAR-γ antibody and an PPAR-γ-HRP conjugate enzyme-linked immunosorbent process assay (ELISA) to determine the concentration of PPAR-γ in serum, plasma, urine, cell culture supernatant and tissue homogenates. Principle of the assay: The sample and buffer…

The hypotheses given were greatly supported during the experiment. Three of the four drugs; Lignocain, Hexamethonium, and Atropine, all showed a decline of inhibition of peristalsis. Nicardipine showed to have complete inhibition of peristalsis. It showed to have a 0 au drug amplitude. There were two standard pressures for this experiment. This was due to the two groups having to compensate for the differences in the two tissues used. Pressures used for Hexamethonium and Nicardipine was 2 cm/H2O…

Effects of Temperature and Substrate on Catalyst Enzymes Ryan Smith Lab Section 030 Abstract In order to turn hydrogen peroxide into water and oxygen by using enzyme called a catalyst. To determine the effects of temperature and substrate concentrations we preformed an experiment testing the effects on the catalyst enzyme reaction. The hypothesis for our first experiment was if the substrate concentration is larger than the catalyst enzymes activity will be faster as well. The result followed…

the reaction rate was measured by the effects of the varying conditions the substrate was in. Influence of Catalase Concentration The higher the concentration of the enzyme catalase, the function of the enzyme occurs better. As the number of disks increased, the concentration increased. Catalase breaks the hydrogen peroxide down quickly at the beginning of the experiment as catalase is a very fast enzyme that breaks down H2O2. The rate of reaction increased and the amount of absolute…

For this catalase experiment it was decided that the group was to keep the concentration of catalase and hydrogen peroxide the same and only change the level of pH buffer throughout the experiments conducted. This was done to observe the effect different levels of pH buffers have on the breakdown of hydrogen peroxide and the volume of oxygen released. The results from the majority of experiments conducted reflect that though they are in consensus with the hypothesis that the volume of oxygen…

does the change of pH affect the rate of decomposition of hydrogen peroxide on the enzyme, catalase? Background Information: Enzymes are globular proteins, which are synthesised by cells in order to perform specific functions. These act as catalysts in chemical reactions and are considered a major factor in an organism as these reaction rates are required to be shortened in order to support life. Catalase is one of the most important enzymes in living organisms, such as animals, plants and…