Catalase

Measuring the Average Catalase Reaction Times of Solanum tuberosum Supernant in Hydrogen Peroxide Julie Fitzgerald Delano Woods Biology 103: Botany Professor Silady, Smith February 23, 2017 INTRODUCTION This investigation is geared towards observing the enzyme reaction times in Solanum tuberosum, also known as the common potato, depending on the temperature of the catalase. During the lab, students tested the reaction times of the catalase when placed in hydrogen peroxide when the…

Concentration on Catalase Reactions Blake Devivo Biol 101 Lab February 10, 2016 Abstract The human body contains thousands of tiny structures called enzymes that perform specific functions. One enzyme called “Catalase” is often used to regulate the amount of a toxic compound, hydrogen peroxide, in the body. The purpose of this experiment was find the different factors that can affect Catalase-Peroxide reactions. The first part of this experiment demonstrated that catalase reactions with…

rather than the predicted optimum temperature of 39oC. Errors within the individual group results, concluded that the both 10oC and 20oC were wrong due to the vast froth volumes. For a smaller amount of catalase, this would result in a smaller froth volume, conversely, with a greater amount of catalase, the froth volume will be greater. Overall, the results found in the combined results did support the hypothesis, but they were not as accurate as predicted. From the results obtained within the…

fact that enzymes break down molecules into smaller pieces. The objective of this lab is to observe how quickly the enzyme catalase breaks down H2O2, and what long periods of contact between the two do to that process. We used seven different cups for seven different lengths of time, and in every cup we mixed 10 mL of 1.5% H2O2, 1 mL of distilled water, and 1 mL of catalase. We then waited the indicated period of times on the cups, which is our independent variable, to put the sulfuric acid…

Gram + Cocci To identify the Gram + cocci I first performed a catalyst test which produced oxygen bubbles and indicated a positive catalase result. The next test I preformed was a mannitol salt test using a mannitol-salt agar plate and the results showed positive fermentation turning the plate yellow and also positive growth. This led me to conclusion that my unknown Gram + cocci was Staphylococcus aureus. Gram – Rod To identify the unknown Gram – rod I first performed a H2S production test…

for the rest of the tests did make sense. For example, the solution contained manganese dioxide and H2O2 reacted. This indicates that catalase enzyme was present in manganese dioxide which is classified as an inorganic compound. When these two substances mixed, H2O2 eventually turned into water and oxygen. The rate of reaction of this…

height of foam after 10 minutes. Freshly-prepared potato juice was used as the enzyme for catalase. Since temperature is measured by the kinetic energy of molecules, the higher the temperature, the more movement of molecules. The lowest temperature makes it more likely the molecules will not come in contact with each other. Introduction The effect of temperature on enzyme activity was presented by enzyme catalase, found in membrane-bound vesicles called peroxides. Research findings suggest that…

tremendous amount of fizzing at 40˚C. The fizzing rate of each test tube helped us draw our conclusions. We were able to identify what temperatures the catalase enzyme activity reached its maximum. We’ve learned that enzyme activity is much slower in colder temperatures and reacts best when certain temperatures are reached. In this case, the catalase enzyme has optimum activity roughly between 25 and 40 degrees celsius. The potato cell was unfortunately denatured at 80˚C, this is because the…

Modeling enzymes: How does catalase affect the initial rate of formation and total amount of O2? Introduction In water, hydrogen peroxide converts naturally into diatomic oxygen gas and water through the collision of water molecules. However, this reaction occurs at an extremely slow rate. With the presence of an enzyme, catalase, the reaction occurs at a much faster rate. This is because catalase binds with the hydrogen peroxide substrate at its transition state to weaken the covalent bonds,…

faster than they would on their own (Lawless 2016). Due to the fact that so many different arrangements of proteins are possible, it’s no surprise that so many reactions in the body are all able to proceed faster with the help of enzymes. In this lab, catalase was used to break down hydrogen peroxide and measure the reaction rates depending on the H2O2 concentration. The purpose of this was to see if the concentration of H2O2 affected the rate of the reaction. The hypothesis tested was that…