Amylase

results of this experiment, bacterial and fungal amylase have different optimal temperatures, which suggests that they function in different environments. Bacterial amylase, Bacillus Licheniformis, has an optimal temperature of 55ºC, while fungal amylase, Aspergillus Oryzae, has an optimal temperature between 25ºC and 55ºC, leaning more towards 55ºC. As we can tell from the results, temperature does have an effect on enzyme activity. As predicted, amylase activity increased until it reached its…

pH and temperature amylase activity from fungal and mammal Introduction The first enzyme that was produced industrially is amylase from a fungal source in 1894, it was used to treat digestive disorder. Amylase is an enzyme that breaks down starch into sugar and starts the process of chemical digestion in human salvia. Starch breakdown of amylase has received a great deal of attention because of their technological significance and economic benefits, and is also used for the commercial…

α-glycosidic linkages in starch are called amylases…

enzyme resides in4. While different enzymes have different optimum pH’s, an extremely high or low pH will often denature an enzyme. Amylase is a frequently extracted enzyme from bacteria, fungi, and yeast2. Amylase has an optimal pH of 12 and temperature ranging from 75-105 °C2. In this experiment, the effects of temperature and pH on the catalytic activity of amylase were…

The table below shows what colour the solution has turned after Amylase solution and Benedict’s solution has been added to Starch solution at different temperatures. The tests were completed three times each temperature to find an average. This graph resembles the results from the table above. Making it obvious to observe the unexpected results as the temperatures 2, 20 and 27 remained blue then 37 degrees changed to green and 60 degrees changed to orange. This experiment was carried out to…

The first stating that if pH increases, then amylase activity will increase. The null of this being if pH increases, then amylase activity will not increase. The final hypothesis is as follows: If temperature increases, then amylase activity will increase. The null of this being if temperature increases, then amylase activity will not increase. To begin with, an thorough background knowledge and understanding of enzymes is imperative…

The Effects of Concentration Changes on Porcine Pancreatic α-Amylase Activity Introduction Starch, a complex polysaccharide comprised of glucose monomers, is used as a storage compound in the plants it is found to be present in. As a result it is a renewable and biodegradable source of food for humans and many animals that devour these plants (Man et al. 2013). In 1831 a discovery was made by Erhard Leuchs that when mixed with human saliva, starch molecules are broken down into simpler…

This used the already gelatinized starch from Experiment 2.1, where amylase is 25% present. The production of saliva, is when α-amylase is secreted to start the digestion process (Bailey and Howard 1963). Glucose, maltose, malotriose are all low molecular weight products that that are formed through hydrolysis of amylase of internal α-1,4 and α-1,6-glycosidic linkages (Fenemma and others 2008). Through gelatinization both amylose and amylopectin…

humans. Though many people believe that zombies are just a creation of fiction, the undead has risen and it is our job to cure or treat the problem at hand. This plague is being caused by Amylase Deficiency Condition, or ADC for short, this is caused by a gene mutation that restricts the production of pancreatic amylase to break down glucose in the body. As it stands now, their are two main methods to combat the zombie epidemic, recombinant DNA or CRISPR. Recombinant DNA or rDNA for short, is…

Enzymes Dmitri N. Piquero Biological Discovery I Laboratory Section I Abstract Amylase is an enzyme that speeds up the conversion rate from starch to glucose. Four test tubes were filled with a starch solution and a different concentration of amylase solution ranging from 0 μL to 1000 μL. The test tubes were then left to soak in a 37°C water bath before having 2 mL of 1 M hydrochloric acid added to a test tube of pure amylase solution. Lugol’s iodine was used to test for the existence of…