Amylase

production of enzymes by the endophytic fungi Amylase activity Amylase enzyme was assessed by growing the fungi on glucose yeast extract peptone (GYP) agar medium (glucose- 1g, yeast extract- 0.1g, agar- 15 g, distilled water 1000mL and pH 6) containing 1 % soluble starch after 5 days incubation , the plates with fungal colony were flooded with 1% iodine and 2% potassium iodide. The appearance of clear zone surrounding the colony was considered positive for amylase enzyme. Protease activity…

of diverse amylase and each has different optimal temperatures in which they complete their reaction faster and for this case the percentage used was 1% bacterial amylase and 3% Fungal was used. In order to find the optimal temperature we had to conduct an experiment on this two amylases. By observing the effect temperature has on the amylases we found the optimal temperature. As the temperature increases the reaction the amylase is undergoing increases therefore the different amylases used in…

with a hypothesis before testing/knowing the results. When amylase influenced starch disappearance, I thought that the greater the amylase percentage in the solution during experiment one, the faster it would react. In experiment two, pH influenced the activity of amylase. My hypothesis was, When the pH level increases, the time of starch disappearance decreases.…

An Investigation into the Activity of Alpha-amylase Enzyme in Relation to Temperature and PH Introduction: The role of amylase in plants is the breakdown of starches so that they can be used as energy in the first stages of early growth (wise geek, [B] 2016). Enzymes are extremely efficient natural catalysts that are found in cells, they work by lowering the activation energy of a reaction and providing an alternative reaction pathway. Enzymes are very specific, only catalysing certain reactions…

The control was the first row for both amylases. At 0 min. neither one had synthesized anything so this served as the starting point. The independent variable was the temperature; this is what was being changed. The dependent variable was the amylase activity; this is what (should have) changed because of the temperature. The iodine was a marker. It showed whether starch was still present in the wells. From the results, starch was present in all the wells; it was present at every temperature and…

Results of the experiment for the fungal amylase activity were in support of previous research; similar to Serrano and Peralta’s (2015) study, optimum amylase activity was clearly indicated in the results. Denaturation was also clearly indicated with regards to fungal amylase. According to the data gathered in our experiment, the optimum range for bacterial amylase activity appears to fall between 25° C and 55° C, with the lowest averages falling in the 55° C column in Table 2. The process of…

Change in Temperature Conditions on Enzymes Bacillus licheniformis Amylase and Aspergillus oryzae Amylase Over Time ____________________________________ Roxanna Flores PID: 5199946 Lab Section U26 Group Members: Roudabyna Taleb Abstract This laboratory was performed in order to be able to determine the effect of temperature over time on bacterial amylase Bacillus licheniformis and fungal amylase Aspergillus oryzae. Bacterial amylase was introduced into four singular test tubes at different…

Question: How does the concentration of the salivary amylase affect its activity? Aim: To investigate the effects of concentration on the activity of salivary amylase Hypothesis: The lower the concentration of the salivary amylase, the less the enzyme activity and vice-versa is true provided that other factors such as temperature and the pH are kept constant. Variables: Independent variables: • the concentration of the salivary amylase Dependent variables: • the enzymatic activity…

experiment was amylase and its corresponding substrate was starch, by binding together amylase was able to break down starch into maltose. The question of interest of this experiment was whether or not temperature would affect the rate of enzymatic activity, this was observed by measuring the amount of starch that was digested by amylase at varying temperatures.…

chosen for the experiment was an amylase, specifically beta-amylase. Amylase protein can be typically found in plants and animals. The role of this protein is to hydrolyze organic materials, such as starch and dextrin, in order to form glucose, maltose, and limit dextrin. One distinction between beta amylase and alpha amylase is that beta amylase is not found in animal. It is found primarily in plants, bacteria, and fungi. In these types of organisms, beta amylase plays a huge role in its…