BIOLOGY LAB REPORT.
ENZYME ACTIVITY
Planning (a) – Pl (a):
Research Question:
How does the concentration of the salivary amylase affect its activity?
Aim:
To investigate the effects of concentration on the activity of salivary amylase
Hypothesis:
The lower the concentration of the salivary amylase, the less the enzyme activity and vice-versa is true provided that other factors such as temperature and the pH are kept constant.
Variables:
Independent variables:
• the concentration of the salivary amylase
Dependent variables:
• the enzymatic activity
• the colour change of the saliva solution
Controlled variables:
• the temperature
• ph
Planning (b) – Pl (b):
Requirements:
1. distilled water
2. 0.5% starch solution
3. buffer …show more content…
I labelled the test tubes according to their concentrations.
6. I transferred 2mL of starch solution into another 4 test tubes and added 2mL of buffer 7.0 to each test tube
7. I placed the two sets of test tubes in a water bath (approximately 37 C) for 5 minutes.
8. I put some IKI in a spotted white tile and noted the time
9. I poured the contents of one tube containing starch into the tube with highest concentration of saliva.
10. I swirled it to mix and returned it quickly to the water bath.
11. I tested the mixed solution for starch by removing a small drop of the reaction mixture with a clean eye dropper and added it to the first drop of iodine in the spotted white tile.
12. I noted the colour.
13. I discarded the mixture in the eye dropper and got a new drop from the test tube. I tested it with the second drop of iodine. It was important to always get a fresh drop of the mixture from the test tube since it cooled rapidly.
14. I repeated the test at 10 second intervals until I reached the endpoint of the reaction, the point at which the mixture remained the same colour as the iodine solution.
15. I repeated this procedure using the other dilutions of saliva. I prepared a new tile when needed.
16. I recorded my
ENZYME ACTIVITY
Planning (a) – Pl (a):
Research Question:
How does the concentration of the salivary amylase affect its activity?
Aim:
To investigate the effects of concentration on the activity of salivary amylase
Hypothesis:
The lower the concentration of the salivary amylase, the less the enzyme activity and vice-versa is true provided that other factors such as temperature and the pH are kept constant.
Variables:
Independent variables:
• the concentration of the salivary amylase
Dependent variables:
• the enzymatic activity
• the colour change of the saliva solution
Controlled variables:
• the temperature
• ph
Planning (b) – Pl (b):
Requirements:
1. distilled water
2. 0.5% starch solution
3. buffer …show more content…
I labelled the test tubes according to their concentrations.
6. I transferred 2mL of starch solution into another 4 test tubes and added 2mL of buffer 7.0 to each test tube
7. I placed the two sets of test tubes in a water bath (approximately 37 C) for 5 minutes.
8. I put some IKI in a spotted white tile and noted the time
9. I poured the contents of one tube containing starch into the tube with highest concentration of saliva.
10. I swirled it to mix and returned it quickly to the water bath.
11. I tested the mixed solution for starch by removing a small drop of the reaction mixture with a clean eye dropper and added it to the first drop of iodine in the spotted white tile.
12. I noted the colour.
13. I discarded the mixture in the eye dropper and got a new drop from the test tube. I tested it with the second drop of iodine. It was important to always get a fresh drop of the mixture from the test tube since it cooled rapidly.
14. I repeated the test at 10 second intervals until I reached the endpoint of the reaction, the point at which the mixture remained the same colour as the iodine solution.
15. I repeated this procedure using the other dilutions of saliva. I prepared a new tile when needed.
16. I recorded my