Quantification of Iron in
Whole Grain Cereal
Jacob Rickman and Yasmeen Coan
Submitted to: Curt Zanelotti
CHEM 1045, Experiment No. 12, April 15, 2016
LABORATORY WRITTEN REPORT
Honor Code Signature: _________________
Objective
In this experiment, we gained an understanding of the relationship between chemistry and food products while developing and enhancing good lab techniques. We applied dilution concepts to carry out the experiment. By using these concepts and techniques, we created a dilution curve to display and understand our data.
Procedure1
Materials
The materials used in this experiment are whole grain cereal, Sodium Acetate, water, hydrochloric acid, 2-2 Dipyridyl solution, Hydroxylamine-HCl, and D.I. water. The glassware and …show more content…
Create a hot water bath by heating a 100mL beaker filled with water. Place the test tube into the hot water bath for about 10 minutes. When hydrogen gas stops forming and the solution is pale green, then stop heating the beaker.
7. Transfer the solution to a 100mL volumetric flask and dilute the solution to 100mL.
8. Create standard Fe2+ solutions (1, 2, 4, 5, and 8 ppm):
a. To create 1 ppm use 1.0mL of a 10ppm Fe 2+ solution into a test tube with a mark at the 10mL line.
b. Add two drops of 6.0M HCl(aq), 0.5mL 10% Hydroxylamine-HCl, six drops 2.0M Sodium Acetate, and 1.0mL 0.1% 2,2-Dipyridyl solution to the test tube.
c. Fill the test tube to the 10mL mark with DI water and the color will fully develop in 15 minutes
d. Prepare the four other standard solutions with appropriate amounts of the solutions.
9. The sample uses 2.0mL from the volumetric flask with the dissolved Fe and add the appropriate reagents.
10. Use an Agilent Atomic Absorption instrument to determine the concentration of Fe+2.
Experimental Data
Sample Concentration (ppm) Absorbance
Standard 1 1 ppm .0470
Standard 2 2 ppm .1788
Standard 3 4 ppm .3232
Standard 4 5 ppm .4124
Standard 5 8 ppm .6053
Unknown Sample .91 ppm .9126
Table 1-1: Experimental …show more content…
Taking the fine powdered cereal and mixing with water, the iron could be extracted because of iron being added to the cereal in elemental form. This is not the most accurate way to collect iron from a sample, but it was mostly successful. The amount of iron collected was poor because of the big percentage of error in this experiment. Using the standards provided in class, a calibration curve could be created to get a general idea of how much iron was in the unknown
Whole Grain Cereal
Jacob Rickman and Yasmeen Coan
Submitted to: Curt Zanelotti
CHEM 1045, Experiment No. 12, April 15, 2016
LABORATORY WRITTEN REPORT
Honor Code Signature: _________________
Objective
In this experiment, we gained an understanding of the relationship between chemistry and food products while developing and enhancing good lab techniques. We applied dilution concepts to carry out the experiment. By using these concepts and techniques, we created a dilution curve to display and understand our data.
Procedure1
Materials
The materials used in this experiment are whole grain cereal, Sodium Acetate, water, hydrochloric acid, 2-2 Dipyridyl solution, Hydroxylamine-HCl, and D.I. water. The glassware and …show more content…
Create a hot water bath by heating a 100mL beaker filled with water. Place the test tube into the hot water bath for about 10 minutes. When hydrogen gas stops forming and the solution is pale green, then stop heating the beaker.
7. Transfer the solution to a 100mL volumetric flask and dilute the solution to 100mL.
8. Create standard Fe2+ solutions (1, 2, 4, 5, and 8 ppm):
a. To create 1 ppm use 1.0mL of a 10ppm Fe 2+ solution into a test tube with a mark at the 10mL line.
b. Add two drops of 6.0M HCl(aq), 0.5mL 10% Hydroxylamine-HCl, six drops 2.0M Sodium Acetate, and 1.0mL 0.1% 2,2-Dipyridyl solution to the test tube.
c. Fill the test tube to the 10mL mark with DI water and the color will fully develop in 15 minutes
d. Prepare the four other standard solutions with appropriate amounts of the solutions.
9. The sample uses 2.0mL from the volumetric flask with the dissolved Fe and add the appropriate reagents.
10. Use an Agilent Atomic Absorption instrument to determine the concentration of Fe+2.
Experimental Data
Sample Concentration (ppm) Absorbance
Standard 1 1 ppm .0470
Standard 2 2 ppm .1788
Standard 3 4 ppm .3232
Standard 4 5 ppm .4124
Standard 5 8 ppm .6053
Unknown Sample .91 ppm .9126
Table 1-1: Experimental …show more content…
Taking the fine powdered cereal and mixing with water, the iron could be extracted because of iron being added to the cereal in elemental form. This is not the most accurate way to collect iron from a sample, but it was mostly successful. The amount of iron collected was poor because of the big percentage of error in this experiment. Using the standards provided in class, a calibration curve could be created to get a general idea of how much iron was in the unknown