Canine Pancreatic Lipase Lab Report

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Skill #28
Kirstin Krueger #21839116 ELISA is an abbreviation for "enzyme-linked immunosorbent assay." An ELISA test uses different parts of the immune system, different antibodies, and color changes to detect immune responses in the body. CPLi, or Canine Pancreatic Lipase Immunoreactivity, is a specific lipase that is only found in canine pancreatic tissues. The CPLi ELISA snap test is made by Idexx and is used to measure the concentrations of pancreatic lipase in the serum of the patient. An antibody elsa test works when a blood sample and the conjugate specific for that test are combined together. The conjugate contains an antibody that has an enzyme connected to it. That enzyme will be what binds to the antigen in the infected sample.
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1.5 mls were placed in a purple top tube containing EDTA for a CBC and the other 1.5mls were placed in a red top tube containing a serum separator.
I then allowed the blood to clot in that tube at room temperature for 30 minutes while my CPL test that is stored in the refrigerator is set out to become room temperature.
Samples must be at room temperature (18–25 C) before beginning the test procedure.
For this test, the serum can either fresh or stored at 2–8 C for up to one week.
After the blood was clotted I gently placed the sample in the centrifuge and spun it down at 2500rpm for 10 minutes.
Once the blood was done spinning, I transferred the serum from the red top tube and placed it in a second small red top tube.
I then labeled it with the date, time of collection, patient 's name, and chart number, and started to prepare the rest of the test. NOTE: Hemolyzed, icteric or lipemic samples will not affect these test results. Test Procedure
Check the lot # on the SNAP package and the lot # on the blue conjugate; they should match. If not, DO NOT
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The word “SNAP” will be facing towards you.
Add the contents of the tube to the circular well of the SNAP test.
Sample will flow up until the entire screen is covered.
This step allows the conjugate/antigen complex to bind to the the antibody.
Once you see the sample come up into the small activator circle, push the test down with the palm of your hand until you hear it snap.
NOTE: Some samples may not flow to the activation circle within 60 seconds. In this case, press the activator after the sample has flowed across the result window or at 60 seconds. Do not let it sit longer than that.
This step allows the sample to flow back across the matrix screen and gives a second chance for the conjugate/antigen to bind with the antibody.
Pick either the RIGHT or LEFT side of the machine and push the SNAP test in (with the LARGE sample well going in first); push until you hear a click.
This step allows a "wash" to go over the matrix to remove any debris that may interfere with the results. A colorless solution then interacts with the enzymes. Each enzyme converts multiple molecules of these subsrates which creates that BLUE color you see on the results

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