Assay

example is protein purification; during protein purification the quantity of protein present must be known to determine the yield1. The quantification of proteins is determined by methods known as protein assays. There are different protein assays used to determine the concentration of proteins. Three different types of protein assays used by biochemists include the Bicinchoninic Acid (BCA) protein assay, Modified Lowry protein assay, and the o-Phthalaldehyde (OPA) Fluorescent Protein…

The acyl-biotinyl exchange assay (ABE assay) is a powerful technique that enables a more sensitive, rapid, less time consuming, more qualitative method of observing protein palmitoylation while allowing to detect small changes There are four basic steps to this process: immunoprecipitation, HAM biotin labeling, Biotin-BMCC treatment: labelling, and purification of the thiol-biotinylated proteins. Prior to ABE assay, a cell culture must be prepared, and proteins must be extracted from the…

The assay was performed as described in section 3.2.2.2. P4 concentrations were analysed in spent culture media for cells cultured for 48, 96 and 144 hr in the present of melatonin 0, 20, 200 and 2000 pg/ml under both 5% O2 and air condition. Samples were diluted 100 fold in PBS and analysed in duplicate with standard (0, 0.5, 1, 2, 5, 10 and 20ng/ml), blank and quality control. To avoid variation among plates, all samples were run together. Inter and intra assay % CV was (11.1) and (5.1)…

Infectious Diseases. 15:523. DOI 10.1186/s12879-015- 1248-6. This article compares and contrasts the sensitivity and specification of Enzym-linked Immunosorbent Assay (ELISA) and the Widal test for diagnostic purposes. To compare the two assays the researchers used blood culture collected from febrile patients for the last three years. The paper clime the widely used Widal testing method has a poor sensitivity as well as up to three days of delayed response time. In addition, the Widal testing…

Skill #28 Kirstin Krueger #21839116 ELISA is an abbreviation for "enzyme-linked immunosorbent assay." An ELISA test uses different parts of the immune system, different antibodies, and color changes to detect immune responses in the body. CPLi, or Canine Pancreatic Lipase Immunoreactivity, is a specific lipase that is only found in canine pancreatic tissues. The CPLi ELISA snap test is made by Idexx and is used to measure the concentrations of pancreatic lipase in the serum of the patient.…

both the assays done. This would be characterized by the readings showing two parallel lines on the graph. However, the two first readings did not demonstrate this. This could possibly be due to different and insufficient amounts of NADH+ being added to the serums, which would mean that the reaction would not occur as fast as normal and at different rates. A third assay was carried out, which was not parallel to either of the previous assays, but most likely to be accurate as the correct amount…

The data show that in separating the sample mix using size-exclusion chromatography, or gel filtration chromatography, the hemoglobin was able to be separated from the vitamin B12 suggesting that the data are consistent with the hypothesis. This is evident in the data since the concentration of protein decreased with each collected fraction after the first fraction that collected the protein, fraction 2, and leveled off. This is shown by the decreasing absorption measurements in Table 3. The…

Rabia Iqbal Biol 395-1005 lab 01/31/2015 Broadford assay Absorbance Figure 1. The concentration and absorbance of Bradford assay. Four samples containing Bovine serum albumin, diluent, and Bradford. At 0.25 mg/ml concentration the absorbance was 0.109 nm and the relation between absorbance and the concentration held constant throughout except at the 0.50 mg/ml when the concentration absorbance was 0.186 nm. Figure showed that as the concentration is increased the absorbance elevates also. All…

The comet assay, also known as single-cell gel electrophoresis (SCGE), is a standard lab technique that can measure deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells. This technique originated in 1984 with the purpose of quantifying the amount of DNA damage in a cell (3). Originally the test was run using neutral conditions, but this only allowed for detection of double stranded DNA breaks. In 1988 the technique was adapted to use alkaline conditions that made it more sensitive and…

subjected the CCR5 ZFN binding site to PCR amplification for 25 cycles. After PCR amplification and gel purification the sequence was subject to a modified Surveyor nuclease assay to determine CCR5 disruption frequency. Surveyor nuclease assays are used to detect single base mismatches or small insertions or deletions. It uses a mismatch-specific endonuclease that recognizes all base substitutions and insertions or deletions. When it finds one of the three, it cleaves the 3’ side of the…