Trend #1: Lateral Flow Immunoassay (LFI)
Shan, S., Lai, W., Xiong, Y., Wei, H., and Xu, H. (2015) Novel Strategies To Enhance Lateral Flow Immunoassay Sensitivity for Detecting Foodborne Pathogens. Journal of Agricultural and Food Chemistry. 63, 745−753. DOI: 10.1021/jf5046415.
This article talks about the simplicity, stability and sensitivity of conventional lateral flow immunoassay (LFI) device for the use of detecting foodborne pathogens. Foodborne pathogen has prevailed to cause disease and even death in individuals that has been exposed to bacteria thru food consumption. Therefore, the researchers in this paper examine, in great detail, how the conventional lateral flow immunoassay can be enhanced for stringent sensitivity as well as …show more content…
A., Rauniyar, R., Raut, p, p., Manandhar, K, D., and Gupta, B, P. (2015) Evaluation of sensitivity and specificity ELISA AGAINST Widal test for typhoid diagnosis in endemic population of Kathmandu. BMC Infectious Diseases. 15:523. DOI 10.1186/s12879-015- 1248-6.
This article compares and contrasts the sensitivity and specification of Enzym-linked Immunosorbent Assay (ELISA) and the Widal test for diagnostic purposes. To compare the two assays the researchers used blood culture collected from febrile patients for the last three years. The paper clime the widely used Widal testing method has a poor sensitivity as well as up to three days of delayed response time. In addition, the Widal testing can easily be influenced by the use of antibiotics resulting in false negatives. In contrast with the ELISA assays, the article climes to have a more accurate, specific result within acceptable time frame. The researchers have a 95% confidence level for ELISA testing compare to 72% confidence level for Widal testing. The researcher recommends replacing the Widal testing system with a more accurate assay like the ELISA assay for diagnostic …show more content…
However, according to this paper, some of the limitations of ELISA based testing are the hourly delay; the time in which antigen binds to the coated antibodies. The excessive use of reagents to coat and wash the 96-well plate, and the 96-well plates it is self where the assay is performed on. Therefore, this article introduces 3D printing platforms for ELISA 96-well plates. With 3D plates, sensitivity of the assay increased as well as antibody binding time to the 96-well plates bay 2 to 3 folds of the traditional ELISA plate would take. This 3D 96-well pate provides a potential use of reduced volume of reagent and samples while increasing sensitivity and time of
Shan, S., Lai, W., Xiong, Y., Wei, H., and Xu, H. (2015) Novel Strategies To Enhance Lateral Flow Immunoassay Sensitivity for Detecting Foodborne Pathogens. Journal of Agricultural and Food Chemistry. 63, 745−753. DOI: 10.1021/jf5046415.
This article talks about the simplicity, stability and sensitivity of conventional lateral flow immunoassay (LFI) device for the use of detecting foodborne pathogens. Foodborne pathogen has prevailed to cause disease and even death in individuals that has been exposed to bacteria thru food consumption. Therefore, the researchers in this paper examine, in great detail, how the conventional lateral flow immunoassay can be enhanced for stringent sensitivity as well as …show more content…
A., Rauniyar, R., Raut, p, p., Manandhar, K, D., and Gupta, B, P. (2015) Evaluation of sensitivity and specificity ELISA AGAINST Widal test for typhoid diagnosis in endemic population of Kathmandu. BMC Infectious Diseases. 15:523. DOI 10.1186/s12879-015- 1248-6.
This article compares and contrasts the sensitivity and specification of Enzym-linked Immunosorbent Assay (ELISA) and the Widal test for diagnostic purposes. To compare the two assays the researchers used blood culture collected from febrile patients for the last three years. The paper clime the widely used Widal testing method has a poor sensitivity as well as up to three days of delayed response time. In addition, the Widal testing can easily be influenced by the use of antibiotics resulting in false negatives. In contrast with the ELISA assays, the article climes to have a more accurate, specific result within acceptable time frame. The researchers have a 95% confidence level for ELISA testing compare to 72% confidence level for Widal testing. The researcher recommends replacing the Widal testing system with a more accurate assay like the ELISA assay for diagnostic …show more content…
However, according to this paper, some of the limitations of ELISA based testing are the hourly delay; the time in which antigen binds to the coated antibodies. The excessive use of reagents to coat and wash the 96-well plate, and the 96-well plates it is self where the assay is performed on. Therefore, this article introduces 3D printing platforms for ELISA 96-well plates. With 3D plates, sensitivity of the assay increased as well as antibody binding time to the 96-well plates bay 2 to 3 folds of the traditional ELISA plate would take. This 3D 96-well pate provides a potential use of reduced volume of reagent and samples while increasing sensitivity and time of