example is protein purification; during protein purification the quantity of protein present must be known to determine the yield1. The quantification of proteins is determined by methods known as protein assays. There are different protein assays used to determine the concentration of proteins. Three different types of protein assays used by biochemists include the Bicinchoninic Acid (BCA) protein assay, Modified Lowry protein assay, and the o-Phthalaldehyde (OPA) Fluorescent Protein…
Then, using the same procedure as before, the solutions ' ultraviolet absorptions at 450 and 595 were determined and recorded in Table 4. As for the BCA assay, 100 microliters of each ovalbumin dilution were combined with 900 microliters of BCA, and the resulting solution were analyzed with a spectrophotometer in order to determine the uv absorbances at 560 nanometers (Data displayed in Table…
The assay was performed as described in section 3.2.2.2. P4 concentrations were analysed in spent culture media for cells cultured for 48, 96 and 144 hr in the present of melatonin 0, 20, 200 and 2000 pg/ml under both 5% O2 and air condition. Samples were diluted 100 fold in PBS and analysed in duplicate with standard (0, 0.5, 1, 2, 5, 10 and 20ng/ml), blank and quality control. To avoid variation among plates, all samples were run together. Inter and intra assay % CV was (11.1) and (5.1)…
Skill #28 Kirstin Krueger #21839116 ELISA is an abbreviation for "enzyme-linked immunosorbent assay." An ELISA test uses different parts of the immune system, different antibodies, and color changes to detect immune responses in the body. CPLi, or Canine Pancreatic Lipase Immunoreactivity, is a specific lipase that is only found in canine pancreatic tissues. The CPLi ELISA snap test is made by Idexx and is used to measure the concentrations of pancreatic lipase in the serum of the patient.…
Infectious Diseases. 15:523. DOI 10.1186/s12879-015- 1248-6. This article compares and contrasts the sensitivity and specification of Enzym-linked Immunosorbent Assay (ELISA) and the Widal test for diagnostic purposes. To compare the two assays the researchers used blood culture collected from febrile patients for the last three years. The paper clime the widely used Widal testing method has a poor sensitivity as well as up to three days of delayed response time. In addition, the Widal testing…
NADH is at 340nm, but the peak absorbance of NAD+ is 259nm (Powers, et al., 2007). Enzyme assays are performed to measure the rate of an enzyme-catalysed reaction, known as the enzyme activity. Many factors can affect the enzyme activity (including temperature, pH, substrate concentration), so all conditions apart from the one being quantified should be standardised. The…
Trypsin is also known to act on itself resulting in both active and inactive forms of trypsin during storage (Sugumaran). In this experiment, affinity chromatography was used to extrapolate the active form of trypsin from the inactive form of trypsin. A column containing P-amino-bezaidine was used to form a bond with the active form of trypsin in the matrix (Sugumaran). Two assays were performed. The first assay was to determine if there was any active trypsin by employing…
zeolite-like structure is well known as the prototype of a number of polynuclear transition-metal hexacyanometalates with the formula, Fe4Ⅲ[FeⅡ(CN)6]3 [1,2]. It exhibits electrochemical [3–5], electrochromic [6–8], photophysical [9,10], and magnetic properties [11,12]. In particular, the electrocatalytic property among these special properties enables the potential analytical application of electrochemical sensor using the PB-modified electrode [13,14]. The reduced form of PB is prussian white…
mixture is made up of only one uniform. The ingredients of an Alka-Seltzer pill consist of 324mg of aspirin, 1625mg of sodium hydrogen carbonate, 965mg of citric acid anhydrous, dimeticone, calcium silicate, docusate sodium, sodium benzoate sodium saccharin, natural and artificial lemon flavor, natural and artificial lime flavor. Each Alka-Seltzer pill has 445mg of sodium and once an Alka-Seltzer is dissolved in water, it turns into bicarbonate which reacts with the hydrogen from the citric acid…
You already know your pool has a pump, but do you really know what that pump actually does? You might be surprised! What Exactly Does a Pool Pump Do? Your pool pump is the heart of your pool and without it, it would die...so to speak. Just like a human heart, your pool's heart (the pump) only has one thing it has to do which is pump water. However, that one thing serves several very important functions, such as: • Pulls the water from your pool through the skimmers and drains. • Then it…